Use of a green fluorescent protein gene as a reporter in Zymomonas mobilis and Halomonas elongata (Journal article)

Douka, E./ Christogianni, A./ Koukkou, A. I./ Afendra, A. S./ Drainas, C.


We investigated the applicability of the green fluorescent protein of Aequorea victoria as a reporter for gene expression in the strictly fermentative Gram-negative ethanologenic bacterium Zymomonas mobilis and in the moderately halophilic bacterium Halomonas elongata. We have succeeded to express a mutated gene of green fluorescent protein under the control of different promoters in Z. mobilis and H. elongata grown under various glucose or salt concentrations, respectively. Our results demonstrate that gfp can serve as an easily assayable reporter gene in both organisms. Maximum fluorescence was obtained in Z. mobilis grown aerobically and in H. elongata grown under elevated salt concentration in solid medium. For both bacteria the fluorescence obtained was higher when the gf gene was placed under the control of a native promoter. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V.. All rights reserved.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: green fluorescent protein,reporter gene,zymomonas mobilis,halomonas elongata,ice nucleation gene,pseudomonas-syringae,expression,plasmid,identification,construction,replication,bacteria,fusions,biology
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/9822
ISSN: 0378-1097
Link: <Go to ISI>://000170159300017
http://onlinelibrary.wiley.com/store/10.1111/j.1574-6968.2001.tb10760.x/asset/j.1574-6968.2001.tb10760.x.pdf?v=1&t=hmsyad2l&s=5d06c8a1b3e070c0a0f218285e0cdd8d2b7ec244
Publisher: Wiley-Blackwell
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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