Use of a green fluorescent protein gene as a reporter in Zymomonas mobilis and Halomonas elongata (Journal article)

Douka, E./ Christogianni, A./ Koukkou, A. I./ Afendra, A. S./ Drainas, C.


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dc.contributor.authorDouka, E.en
dc.contributor.authorChristogianni, A.en
dc.contributor.authorKoukkou, A. I.en
dc.contributor.authorAfendra, A. S.en
dc.contributor.authorDrainas, C.en
dc.date.accessioned2015-11-24T16:52:04Z-
dc.date.available2015-11-24T16:52:04Z-
dc.identifier.issn0378-1097-
dc.identifier.urihttp://olympias.lib.uoi.gr/jspui/handle/123456789/9822-
dc.rightsDefault Licence-
dc.subjectgreen fluorescent proteinen
dc.subjectreporter geneen
dc.subjectzymomonas mobilisen
dc.subjecthalomonas elongataen
dc.subjectice nucleation geneen
dc.subjectpseudomonas-syringaeen
dc.subjectexpressionen
dc.subjectplasmiden
dc.subjectidentificationen
dc.subjectconstructionen
dc.subjectreplicationen
dc.subjectbacteriaen
dc.subjectfusionsen
dc.subjectbiologyen
dc.titleUse of a green fluorescent protein gene as a reporter in Zymomonas mobilis and Halomonas elongataen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.primaryDOI 10.1111/j.1574-6968.2001.tb10760.x-
heal.identifier.secondary<Go to ISI>://000170159300017-
heal.identifier.secondaryhttp://onlinelibrary.wiley.com/store/10.1111/j.1574-6968.2001.tb10760.x/asset/j.1574-6968.2001.tb10760.x.pdf?v=1&t=hmsyad2l&s=5d06c8a1b3e070c0a0f218285e0cdd8d2b7ec244-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.publicationDate2001-
heal.abstractWe investigated the applicability of the green fluorescent protein of Aequorea victoria as a reporter for gene expression in the strictly fermentative Gram-negative ethanologenic bacterium Zymomonas mobilis and in the moderately halophilic bacterium Halomonas elongata. We have succeeded to express a mutated gene of green fluorescent protein under the control of different promoters in Z. mobilis and H. elongata grown under various glucose or salt concentrations, respectively. Our results demonstrate that gfp can serve as an easily assayable reporter gene in both organisms. Maximum fluorescence was obtained in Z. mobilis grown aerobically and in H. elongata grown under elevated salt concentration in solid medium. For both bacteria the fluorescence obtained was higher when the gf gene was placed under the control of a native promoter. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V.. All rights reserved.en
heal.publisherWiley-Blackwellen
heal.journalNameFEMS Microbiol Letten
heal.journalTypepeer reviewed-
heal.fullTextAvailabilityTRUE-
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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