Release of cell-free ice nuclei from Halomonas elongata expressing the ice nucleation gene inaZ of Pseudomonas syringae (Journal article)

Tegos, G./ Vargas, C./ Perysinakis, A./ Koukkou, A. I./ Christogianni, A./ Nieto, J. J./ Ventosa, A./ Drainas, C.


Release of ice nuclei in the growth medium of recombinant Halomonas elongata cells expressing the inaZ gene of Pseudomonas syringae was studied in an attempt to produce cell-free active ice nuclei for biotechnological applications. Cell-free ice nuclei were not retained by cellulose acetate filters of 0.2 mum pore size. Highest activity of cell-free ice nuclei was obtained when cells were grown in low salinity (0.5-5% NaCl, w/v). Freezing temperature threshold, estimated to be below - 7 degreesC indicating class C nuclei, was not affected by medium salinity. Their density, as estimated by Percoll density centrifugation, was 1.018 +/- 0.002 g ml(-1) and they were found to be free of lipids. Ice nuclei are released in the growth medium of recombinant H. elongata cells probably because of inefficient anchoring of the ice-nucleation protein aggregates in the outer membrane. The ice(+) recombinant H. elongata cells could be useful for future use as a source of active cell-free ice nucleation protein.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: moderately halophilic eubacteria,escherichia-coli,biotechnological applications,erwinia-herbicola,reporter gene,protein,identification,principles,repetition,plasmid
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/9063
ISSN: 1364-5072
Link: <Go to ISI>://000165499800008
http://onlinelibrary.wiley.com/store/10.1046/j.1365-2672.2000.01180.x/asset/j.1365-2672.2000.01180.x.pdf?v=1&t=h0dxi3tf&s=4e8642c12643e5373c988c1965e0d87d0ca479a6
Publisher: Wiley-Blackwell
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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