Release of cell-free ice nuclei from Halomonas elongata expressing the ice nucleation gene inaZ of Pseudomonas syringae (Journal article)

Tegos, G./ Vargas, C./ Perysinakis, A./ Koukkou, A. I./ Christogianni, A./ Nieto, J. J./ Ventosa, A./ Drainas, C.

Release of ice nuclei in the growth medium of recombinant Halomonas elongata cells expressing the inaZ gene of Pseudomonas syringae was studied in an attempt to produce cell-free active ice nuclei for biotechnological applications. Cell-free ice nuclei were not retained by cellulose acetate filters of 0.2 mum pore size. Highest activity of cell-free ice nuclei was obtained when cells were grown in low salinity (0.5-5% NaCl, w/v). Freezing temperature threshold, estimated to be below - 7 degreesC indicating class C nuclei, was not affected by medium salinity. Their density, as estimated by Percoll density centrifugation, was 1.018 +/- 0.002 g ml(-1) and they were found to be free of lipids. Ice nuclei are released in the growth medium of recombinant H. elongata cells probably because of inefficient anchoring of the ice-nucleation protein aggregates in the outer membrane. The ice(+) recombinant H. elongata cells could be useful for future use as a source of active cell-free ice nucleation protein.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: moderately halophilic eubacteria,escherichia-coli,biotechnological applications,erwinia-herbicola,reporter gene,protein,identification,principles,repetition,plasmid
ISSN: 1364-5072
Link: <Go to ISI>://000165499800008
Publisher: Wiley-Blackwell
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

Files in This Item:
File Description SizeFormat 
Tegos-2000-Release of cell-free.pdf242.37 kBAdobe PDFView/Open    Request a copy

 Please use this identifier to cite or link to this item:
  This item is a favorite for 0 people.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.