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  5. Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά). ΧΗΜ
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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/9063
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dc.contributor.authorTegos, G.en
dc.contributor.authorVargas, C.en
dc.contributor.authorPerysinakis, A.en
dc.contributor.authorKoukkou, A. I.en
dc.contributor.authorChristogianni, A.en
dc.contributor.authorNieto, J. J.en
dc.contributor.authorVentosa, A.en
dc.contributor.authorDrainas, C.en
dc.date.accessioned2015-11-24T16:46:22Z-
dc.date.available2015-11-24T16:46:22Z-
dc.identifier.issn1364-5072-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/9063-
dc.rightsDefault Licence-
dc.subjectmoderately halophilic eubacteriaen
dc.subjectescherichia-colien
dc.subjectbiotechnological applicationsen
dc.subjecterwinia-herbicolaen
dc.subjectreporter geneen
dc.subjectproteinen
dc.subjectidentificationen
dc.subjectprinciplesen
dc.subjectrepetitionen
dc.subjectplasmiden
dc.titleRelease of cell-free ice nuclei from Halomonas elongata expressing the ice nucleation gene inaZ of Pseudomonas syringaeen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.secondary<Go to ISI>://000165499800008-
heal.identifier.secondaryhttp://onlinelibrary.wiley.com/store/10.1046/j.1365-2672.2000.01180.x/asset/j.1365-2672.2000.01180.x.pdf?v=1&t=h0dxi3tf&s=4e8642c12643e5373c988c1965e0d87d0ca479a6-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.publicationDate2000-
heal.abstractRelease of ice nuclei in the growth medium of recombinant Halomonas elongata cells expressing the inaZ gene of Pseudomonas syringae was studied in an attempt to produce cell-free active ice nuclei for biotechnological applications. Cell-free ice nuclei were not retained by cellulose acetate filters of 0.2 mum pore size. Highest activity of cell-free ice nuclei was obtained when cells were grown in low salinity (0.5-5% NaCl, w/v). Freezing temperature threshold, estimated to be below - 7 degreesC indicating class C nuclei, was not affected by medium salinity. Their density, as estimated by Percoll density centrifugation, was 1.018 +/- 0.002 g ml(-1) and they were found to be free of lipids. Ice nuclei are released in the growth medium of recombinant H. elongata cells probably because of inefficient anchoring of the ice-nucleation protein aggregates in the outer membrane. The ice(+) recombinant H. elongata cells could be useful for future use as a source of active cell-free ice nucleation protein.en
heal.publisherWiley-Blackwellen
heal.journalNameJ Appl Microbiolen
heal.journalTypepeer reviewed-
heal.fullTextAvailabilityTRUE-
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