HILIC separation and quantitation of water-soluble vitamins using diol column (Journal article)

Karatapanis, A. E./ Flamegos, Y. C./ Stalikas, C. D.


Hydrophilic interaction liquid-chromatography (HILIC) in conjunction with diode array detection has been applied for the separation of selected-water-soluble vitamins using an end-capped HILIC-diol column. Vitamins with significant biological importance, such as thiamine (B(1)), riboflavin (B(2)), nicotinic acid (B(3)), nicotinamide (B(3)), pyridoxine (B(6)), folic acid (B(9)), cyanocobalamin (B(12)) and ascorbic acid (vitamin C) were simultaneously separated. Chromatographic conditions including type and percentage of organic modifier in the mobile phase, pH, type and concentration of buffer salt and flow rate were investigated. ACN was shown to offer superior separation for the compounds tested as compared to methanol, isopropanol and THE Isocratic separation and analysis were achieved for six vitamins (B(1), B(2), nicotinic acid/nicotinamide, B(6) and C) at ACN-H(2)O 90:10, containing ammonium acetate 10 mM, triethylamine 20 mM, pH 5.0, using a flow rate of 0.8 mL/min, while a gradient was necessary to resolve a mixture of all eight water-soluble vitamins. The HILIC method was validated and successfully applied to the analysis of a pharmaceutical formulation and an energy drink negating the need for time consuming clean-up steps.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: hydrophilic interaction liquid-chromatography,separation,quantitation,water-soluble vitamins,performance liquid-chromatography,hydrophilic-interaction chromatography,fortified food-products,solid-phase extraction,capillary-electrophoresis,fluorescence detection,dietary-supplements,ionizable compounds,acid-extraction,polar compounds
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/8112
ISSN: 1615-9306
Link: <Go to ISI>://000265123100001
http://onlinelibrary.wiley.com/store/10.1002/jssc.200800525/asset/909_ftp.pdf?v=1&t=h0e1zhku&s=8ba81aef2a3092c0c5cacb28ea46ee66232c57fe
Publisher: WILEY-VCH Verlag
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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