HILIC separation and quantitation of water-soluble vitamins using diol column (Journal article)

Karatapanis, A. E./ Flamegos, Y. C./ Stalikas, C. D.

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dc.contributor.authorKaratapanis, A. E.en
dc.contributor.authorFlamegos, Y. C.en
dc.contributor.authorStalikas, C. D.en
dc.rightsDefault Licence-
dc.subjecthydrophilic interaction liquid-chromatographyen
dc.subjectwater-soluble vitaminsen
dc.subjectperformance liquid-chromatographyen
dc.subjecthydrophilic-interaction chromatographyen
dc.subjectfortified food-productsen
dc.subjectsolid-phase extractionen
dc.subjectfluorescence detectionen
dc.subjectionizable compoundsen
dc.subjectpolar compoundsen
dc.titleHILIC separation and quantitation of water-soluble vitamins using diol columnen
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.primaryDOI 10.1002/jssc.200800525-
heal.identifier.secondary<Go to ISI>://000265123100001-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.abstractHydrophilic interaction liquid-chromatography (HILIC) in conjunction with diode array detection has been applied for the separation of selected-water-soluble vitamins using an end-capped HILIC-diol column. Vitamins with significant biological importance, such as thiamine (B(1)), riboflavin (B(2)), nicotinic acid (B(3)), nicotinamide (B(3)), pyridoxine (B(6)), folic acid (B(9)), cyanocobalamin (B(12)) and ascorbic acid (vitamin C) were simultaneously separated. Chromatographic conditions including type and percentage of organic modifier in the mobile phase, pH, type and concentration of buffer salt and flow rate were investigated. ACN was shown to offer superior separation for the compounds tested as compared to methanol, isopropanol and THE Isocratic separation and analysis were achieved for six vitamins (B(1), B(2), nicotinic acid/nicotinamide, B(6) and C) at ACN-H(2)O 90:10, containing ammonium acetate 10 mM, triethylamine 20 mM, pH 5.0, using a flow rate of 0.8 mL/min, while a gradient was necessary to resolve a mixture of all eight water-soluble vitamins. The HILIC method was validated and successfully applied to the analysis of a pharmaceutical formulation and an energy drink negating the need for time consuming clean-up steps.en
heal.publisherWILEY-VCH Verlagen
heal.journalNameJ Sep Scien
heal.journalTypepeer reviewed-
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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