Adsorption of major endoglucanase from Thermoascus aurantiacus on cellulosic substrates (Journal article)

Mamma, D./ Hatzinikolaou, D./ Kekos, D./ Stamatis, H./ Kalogeris, E.

A thermostable endoglucanase (EndoI) was produced by the thermophilic fungus Thermoascus aurantiacus when grown on cellulosic materials under submerged culture (SC) and solid-state fermentation (SSF). In both cultivation techniques a considerable amount of enzyme activity remained adsorbed onto solid particles, and this was taken into consideration when modeling enzyme production. The results were compatible with the assumption that, following its synthesis, an amount of EndoI was bound on substrate and gradually released into the liquid medium. Adsorption of the enzyme on crystalline cellulose was confirmed in vitro by experiments with purified endoglucanase, which was isolated by anion exchange chromatography. The Langmuir isotherm could efficiently describe the adsorption kinetics, and the estimated A (max) and K (ad) values compared with those obtained for cellulases bearing a binding domain. EndoI displayed high affinity for crystalline cellulose and low binding capacity, which could be beneficial in textile processing.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών
Keywords: endoglucanase,thermoascus aurantiacus,cellulose,adsorption,solid-state fermentation,trichoderma-reesei,cellulase production,culture-conditions,kinetic-model,enzymes,biotechnology,purification,degradation,performance
ISSN: 0959-3993
Link: <Go to ISI>://000264970200006
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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