Adsorption of major endoglucanase from Thermoascus aurantiacus on cellulosic substrates (Journal article)

Mamma, D./ Hatzinikolaou, D./ Kekos, D./ Stamatis, H./ Kalogeris, E.

Full metadata record
DC FieldValueLanguage
dc.contributor.authorMamma, D.en
dc.contributor.authorHatzinikolaou, D.en
dc.contributor.authorKekos, D.en
dc.contributor.authorStamatis, H.en
dc.contributor.authorKalogeris, E.en
dc.date.accessioned2015-11-24T16:34:15Z-
dc.date.available2015-11-24T16:34:15Z-
dc.identifier.issn0959-3993-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/7780-
dc.rightsDefault Licence-
dc.subjectendoglucanaseen
dc.subjectthermoascus aurantiacusen
dc.subjectcelluloseen
dc.subjectadsorptionen
dc.subjectsolid-state fermentationen
dc.subjecttrichoderma-reeseien
dc.subjectcellulase productionen
dc.subjectculture-conditionsen
dc.subjectkinetic-modelen
dc.subjectenzymesen
dc.subjectbiotechnologyen
dc.subjectpurificationen
dc.subjectdegradationen
dc.subjectperformanceen
dc.titleAdsorption of major endoglucanase from Thermoascus aurantiacus on cellulosic substratesen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.primaryDOI 10.1007/s11274-008-9949-2-
heal.identifier.secondary<Go to ISI>://000264970200006-
heal.identifier.secondaryhttp://www.springerlink.com/content/83428318633u25x2/fulltext.pdf-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιώνel
heal.publicationDate2009-
heal.abstractA thermostable endoglucanase (EndoI) was produced by the thermophilic fungus Thermoascus aurantiacus when grown on cellulosic materials under submerged culture (SC) and solid-state fermentation (SSF). In both cultivation techniques a considerable amount of enzyme activity remained adsorbed onto solid particles, and this was taken into consideration when modeling enzyme production. The results were compatible with the assumption that, following its synthesis, an amount of EndoI was bound on substrate and gradually released into the liquid medium. Adsorption of the enzyme on crystalline cellulose was confirmed in vitro by experiments with purified endoglucanase, which was isolated by anion exchange chromatography. The Langmuir isotherm could efficiently describe the adsorption kinetics, and the estimated A (max) and K (ad) values compared with those obtained for cellulases bearing a binding domain. EndoI displayed high affinity for crystalline cellulose and low binding capacity, which could be beneficial in textile processing.en
heal.journalNameWorld Journal of Microbiology & Biotechnologyen
heal.journalTypepeer reviewed-
heal.fullTextAvailabilityTRUE-
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

Files in This Item:
File Description SizeFormat 
Mamma-2009-Adsorption of major.pdf288.38 kBAdobe PDFView/Open    Request a copy


 Please use this identifier to cite or link to this item:
https://olympias.lib.uoi.gr/jspui/handle/123456789/7780
  This item is a favorite for 0 people.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.