Electron microscopic and biochemical study of the effects of rapamycin on glycogen autophagy in the newborn rat liver (Journal article)

Kalamidas, S. A./ Kondomerkos, D. J./ Kotoulas, O. B./ Hann, A. C.


The effects of rapamycin on glycogen autophagy in the newborn rat liver were studied using biochemical determinations, electron microscopy, and morphometric analysis. Rapamycin increased the fractional volume of hepatocytic autophagic vacuoles, the liver lysosomal glycogen-hydrolyzing activity of acid glucosidase, the degradation of glycogen inside the autophagic vacuoles, and decreased the activity of acid mannose 6-phosphatase. These findings suggest that rapamycin, a known inhibitor of the mammalian target of rapamycin (mTOR) signaling, induces glycogen autophagy in the newborn rat hepatocytes. mTOR may participate in the regulation of this process.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Animals,Animals, Newborn,Autophagy/*drug effects,Immunosuppressive Agents/*pharmacology,Liver/*drug effects/metabolism/ultrastructure,Liver Glycogen/*metabolism,Microscopy, Electron,Rats,Sirolimus/*pharmacology
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/22008
ISSN: 1059-910X
Link: http://www.ncbi.nlm.nih.gov/pubmed/14988919
http://onlinelibrary.wiley.com/store/10.1002/jemt.20032/asset/20032_ftp.pdf?v=1&t=h1ym1093&s=cb91cbe5aca4fdc82432ccfd7ef36ef47297a134
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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