Betulinic acid-mediated inhibitory effect on hepatitis B virus by suppression of manganese superoxide dismutase expression (Journal article)

Yao, D. C./ Li, H. W./ Gou, Y. L./ Zhang, H. M./ Vlessidis, A. G./ Zhou, H. Y./ Evmiridis, N. P./ Liu, Z. X.

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dc.contributor.authorYao, D. C.en
dc.contributor.authorLi, H. W.en
dc.contributor.authorGou, Y. L.en
dc.contributor.authorZhang, H. M.en
dc.contributor.authorVlessidis, A. G.en
dc.contributor.authorZhou, H. Y.en
dc.contributor.authorEvmiridis, N. P.en
dc.contributor.authorLiu, Z. X.en
dc.rightsDefault Licence-
dc.subjectpulsatilla chinensisen
dc.subjectreactive oxygen speciesen
dc.subjectelement-binding proteinen
dc.subjecttransgenic miceen
dc.subjectsomatostatin geneen
dc.subjectoxidative stressen
dc.subjectredox regulationen
dc.subjectprotective roleen
dc.subjecthuman liveren
dc.titleBetulinic acid-mediated inhibitory effect on hepatitis B virus by suppression of manganese superoxide dismutase expressionen
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.primaryDOI 10.1111/j.1742-4658.2009.06988.x-
heal.identifier.secondary<Go to ISI>://000264882700016-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.abstractThe betulinic acid (BetA) purified from Pulsatilla chinensis (PC) has been found to have selective inhibitory effects on hepatitis B virus (HBV). In hepatocytes from HBV-transgenic mice, we showed that BetA substantially inhibited HBV replication by downregulation of manganese superoxide dismutase (SOD2) expression, with subsequent reactive oxygen species generation and mitochondrial dysfunction. Also, the HBV X protein (HBx) is suppressed and translocated into the mitochondria followed by cytochrome c release. Further investigation revealed that SOD2 expression was suppressed by BetA-induced cAMP-response element-binding protein dephosphorylation at Ser133, which subsequently prevented SOD2 transcription through the cAMP-response element-binding protein-binding motif on the SOD2 promoter. SOD2 overexpression abolished the inhibitory effect of BetA on HBV replication, whereas SOD2 knockdown mimicked this effect, indicating that BetA-mediated HBV clearance was due to modulation of the mitochondrial redox balance. This observation was further confirmed in HBV-transgenic mice, where both BetA and PC crude extracts suppressed SOD2 expression, with enhanced reactive oxygen species generation in liver tissues followed by substantial HBV clearance. We conclude that BetA from PC could be a good candidate for anti-HBV drug development.en
heal.journalNameFebs Journalen
heal.journalTypepeer reviewed-
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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