Autoantibodies to lipids in bronchoalveolar lavage fluid of patients with acute respiratory distress syndrome (Journal article)

Maneta-Peyret, L./ Kitsiouli, E./ Lekka, M./ Nakos, G./ Cassagne, C.


Objective: To investigate the presence of autoantibodies to lipids in the bronchoalveolar lavage (BAL) fluid from adult patients with acute respiratory distress syndrome (ARDS). Design: Analysis of immunoglobulin G (IgG) in BAL fluid by electrophoresis followed by immunoblotting and characterization of immunoglobulins as antilipid autoantibodies. Setting: Intensive care unit of a university hospital and two research university laboratories. Subjects: Twenty-seven mechanically ventilated patients in total, including nine patients with ARDS and two control groups. Interventions: Patients were ventilated with a mechanical ventilation mode. Six aliquots of 20-mL sterile normal saline at 37 degreesC were infused through the working channel of the bronchoscope. Measurements: Total protein, detection of IgG by electrophoresis followed by immunoblotting, and characterization of IgG by enzyme-linked immunosorbent assay using different lipids as target antigens. Main Results: Antiphospholipid autoantibodies are present in BAL fluid of ARDS patients. Among the phospholipids tested, phosphatidic acid and phosphatidylserine gave the most significant activity. The IgG fraction, purified from BAL fluids by affinity chromatography, gave the same pattern of binding as that of the BAL fluid. Conclusion: The presence of anti phospholipid autoantibodies in BAL fluid suggests involvement of autoimmune mechanisms in the pathogenesis of ARDS.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: antibodies,phospholipids,pulmonary disease,bronchoalveolar lavage fluid,antiphospholipid antibody syndrome,systemic lupus-erythematosus,phospholipids,proteins,ards
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/9945
ISSN: 0090-3493
Link: <Go to ISI>://000171751400017
Publisher: Lippincott, Williams & Wilkins
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

Files in This Item:
File Description SizeFormat 
Lekka-2001-Autoantibodies to lipids.pdf169.47 kBAdobe PDFView/Open    Request a copy



 Please use this identifier to cite or link to this item:
http://olympias.lib.uoi.gr/jspui/handle/123456789/9945
  This item is a favorite for 0 people.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.