Arthrobacter phenanthrenivorans sp nov., to accommodate the phenanthrene-degrading bacterium Arthrobacter sp strain Sphe3 (Journal article)

Kallimanis, A./ Kavakiotis, K./ Perisynakis, A./ Sproer, C./ Pukall, R./ Drainas, C./ Koukkou, A. I.


A novel phenanthrene-degrading bacterium, designated strain Sphe3, was isolated from a creosote-contaminated soil in Greece. Cells were non-motile, Gram-positive, aerobic, and rod- to coccus-shaped. The strain was isolated on the basis of formation of a clear zone on agar plates sprayed with phenanthrene. Optimal growth occurred at 30 degrees C. The G+C content of the DNA was 65.7 mol%. The polar lipid pattern of strain Sphe3 (T) consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The dominant fatty acids were iso-C(15:0), anteiso-C(15:0), iso-C(16:0), C(16:0) and anteiso-C(17:0), representing > 86% of the total fatty acids. The predominant isoprenoid quinone of strain Sphe3(T) was menaquinone-8 (MK-8). Based on 16S rRNA gene sequence analysis, strain Sphe3(T) showed 99 and 98.9% similarity to the type strains of Arthrobacter oxydans and Arthrobacter polychromogenes, respectively. Strain Sphe3(T) showed 91% similarity to homologues of A. oxydans and A. polychromogenes based on recA gene sequence analysis. Based on 16S rRNA and recA gene sequence analysis and DNA-DNA hybridization analysis, as well as physiological and chemotaxonomic characteristics, it is concluded that strain Sphe3(T) represents a novel species of the genus Arthrobacter, for which the name Arthrobacter phenanthrenivorans sp. nov. is proposed. The type strain is Sphe3(T) (=DSM 18606(T)=LMG 23796(T)).
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: polycyclic aromatic-hydrocarbons,sequence alignment,identification,degradation,genetics,DNA,kp7
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/9921
ISSN: 1466-5026
Link: <Go to ISI>://000263576500011
http://ijs.sgmjournals.org/content/59/2/275.full.pdf
Publisher: Society for General Microbiology
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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