Use of sequential oligopeptide carriers (SOCn) in the design of potent Leishmania gp63 immunogenic peptides (Journal article)

Tsikaris, V./ Sakarellos, C./ Sakarellos-Daitsiotis, M./ Cung, M. T./ Marraud, M./ Konidou, G./ Tzinia, A./ Soteriadou, K. P.

The antigenic sequence (250-257) Ac-IASRYDQL (gp63-SRYD) of the major surface glycoprotein of leishmania, gp63, was covalently attached to the Lys-(NH2)-H-E groups of a new sequential oligopeptide carrier (SOCn), namely; (Lys-Aib-Gly)(n) (n=5,6), in order to obtain potent immunogens and sire-specific antibodies. It was shown, using H-1-NMR spectroscopy; that the gp63-SRYD, octapeptides bound to the SOCn retain their original structural profile outlined by an ionic interaction between R and D side chains and a type I beta-turn involving the QNH --> RCO hydrogen bonding Also, the gp63-SRYD octapeptides linked to the carrier do not experience conformational restrictions, probably because of the favorable conformation of the SOCn. Immunizations of outbred rabbits with the peptide carriers designed resulted in high-titered antibody response to the gp63-SRYD octa-peptide and the gp63 cognate protein. Thus, this chemically defined model may be used for incorporating ''protective'' Leishmania epitopes and ultimately for the design of a multivalent synthetic vaccine against leishmaniosis.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: antibodies,fibronectin,vaccine,antigen,surface
ISSN: 1040-5704
Link: <Go to ISI>://A1996WC33700005
Publisher: Eaton Publishing
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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