Ultrasonic-assisted derivatization reaction of amino acids prior to their determination in urine by using single-drop microextraction in conjunction with gas chromatography (Journal article)

Fiamegos, Y. C./ Nanos, C. G./ Stalikas, C. D.

A derivatization-extraction method that avoids tedious preconcentration steps is established in order to determine amino acids accurately at nanogram levels. The method involves conversion of the analytes of concern to N(O,S)-ethoxycarbonyl amino acid ethyl esters and subsequent extraction by single-drop microextraction (SDME) followed by GC analysis. The reaction proceeds smoothly and rapidly under ultrasonication which removes the bubbles from the bulk solution. Precision is acceptable and 12 non-hydrolyzed amino acids can be determined in urine in this manner. As long as the extraction conditions are consistently applied, quantitative analysis can be performed accurately. The limits of detection were satisfactory in the range 0.010-0.025 mug/ml for GC-FID and 0.26-68 ng/ml for GC-MS(SIM) with 1 ml sample volume. (C) 2004 Elsevier B.V. All rights reserved.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: amino acids,ethylchloroformate,derivatization,urine,single-drop microextraction,gc,performance liquid-chromatography,mass-spectrometry,capillary-electrophoresis,solvent microextraction,flow-injection,derivatives,samples,ionization,extraction,proteins
URI: https://olympias.lib.uoi.gr/jspui/handle/123456789/9803
ISSN: 1570-0232
Link: <Go to ISI>://000225413200010
http://ac.els-cdn.com/S1570023204007470/1-s2.0-S1570023204007470-main.pdf?_tid=ec76dfa6791f728baf9759077c46a46c&acdnat=1333040731_95684068eba08c9165befcde4429556b
Publisher: Elsevier
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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