The position of the LysN(epsilon)H(2)-grafted antigens along the sequential oligopeptide carrier, Ac-(Aib-Lys-Aib-Gly)(n) (SOCn-II), influences the antibody recognition: Application to the Sm main autoimmune epitope (Journal article)

Alexopoulos, C./ Tsikaris, V./ Rizou, C./ Sakarellos-Daitsiotis, M./ Sakarellos, C./ Cung, M. T./ Marraud, M./ Vlachoyiannopoulos, P. G./ Moutsopoulos, H. M.

A sequential oligopeptide carrier of antigenic peptides is presented, incorporating two Aib residues in each repetitive moiety: Ac-(Aib-Lys-Aib-Gly)(n), (SOCn-II; n = 2-4). The conformational study, by H-1-nmr, CD, and Fourier transform ir spectroscopy; indicated that the SOCn-II carrier displays a pronounced 3(10)-helix, compared to the Ac-(Lys-Aib-Gly)(n) (SOCn-I) carrier of the same approximately backbone length, previously reported One of the dominant autoimmune epitopes of the Sm and U1RNP cellular components, the PPGMRPP sequence, was coupled to the Lys-(NH2)-H-epsilon groups of the SOCn-II carrier and used as antigenic substrate for detecting anti-Sm/ U1RNP autoantibodies in ELISA assays. Anti-Sm antibodies are highly specific for systemic lupus erythematosus, while anti-U1RNP are specific for mixed connective tissue disease. The anti-(PPGMRPP)(5)-SOCn-II ELISA was compared with the anti-(PPGMRPP)(n)-SOCn-I ELISA, provided that both antigenic substrates possess the same amount of the epitope replicates. The significance of the lysine positions along the oligopeptide backbone of the carrier for a favorable antibody recognition of the anchored antigens is also examined. (C) 2000 John Wiley & Sons, Inc. Biopoly 54: 1-10, 2000.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: sequential oligopeptide carrier,antigenic peptides,socn,sm antigen,3(10)-helix,circular-dichroism spectra,synthetic-peptide,repetitive epitope,alpha-helices,amino-acids,spectroscopy,specificity,secondary,protein,design
ISSN: 0006-3525
Link: <Go to ISI>://000087289500001<1::AID-BIP10>3.0.CO;2-7/asset/10_ftp.pdf?v=1&t=h0e0ip2g&s=5cb0295ab7c8a3bd303ef0df6736f300becfb4e2
Publisher: Wiley
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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