The PON1 M55L gene polymorphism is associated with reduced HDL-associated PAF-AH activity (Journal article)

Kakafika, A. I./ Xenofontos, S./ Tsimihodimos, V./ Tambaki, A. P./ Lourida, E. S./ Kalaitzidis, R./ Cariolou, M. A./ Elisaf, M./ Tselepis, A. D.

The platelet-activating factor acetylhydrolase activity associated with high density lipoprotein (HDL-PAF-AH) may substantially contribute to the antioxidant, antiinflammatory, and overall antiatherogenic effects of HDL. Two enzymes associated with HDL express PAF-AH catalytic activity, PAF-AH itself and paraoxonase-1 (PON1). The relative contribution of these enzymes in the expression of PAF-AH activity on HDL remains to be established. We investigated whether the PON1 polymorphisms (M55L and Q192R) or the PAF-AH polymorphism V379A could affect the PAF-AH activity associated with HDL in both normolipidemic and dyslipidemic (type IIA and IIB) populations. We show for the first time that the PON1 M55L polymorphism significantly affects the HDI-PAF-AH activity in all studied groups, the PON1 L55L individuals having lower enzyme activity compared to those having 1 M and 2 M alleles. No differences in the HDL content concerning the major apolipoprotein and lipid constituents were observed between individuals carrying the PON1 L55L and those with the M55M polymorphism. jlr Our results provide evidence that PON1 significantly contributes to the pool of HDL-PAF-AH activity in human plasma, and suggest that the low PAF-AH activity in HDL carrying the PON1 L alloenzyme may be an important factor contributing to the low efficiency of this HDL in protecting LDL against lipid peroxidation.-Kakafika, A. I., S. Xenofontos, V Tsimihodimos, A. P. Tambaki, E. S. Lourida, R. Kalaitzidis, M. A. Cariolou, M. Elisaf, and A. D. Tselepis. The PON1 M55L gene polymorphism is associated with reduced HDL-associated PAF-AH activity. J. Lipid Res. 2003.44:1919-1926.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: high density lipoprotein,platelet-activating factor acetylhydrolase,paraoxonase-1,pon1 polymorphisms,platelet-activating factor acetyllrydrolase polymorphisms,platelet-activating-factor,high-density-lipoprotein,coronary-heart-disease,independent risk-factor,factor-acetylhydrolase,oxidative modification,missense mutation,paraoxonase gene,artery disease,human-plasma
ISSN: 0022-2275
Link: <Go to ISI>://000186043900012
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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