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  5. Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά). ΧΗΜ
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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/9621
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dc.contributor.authorRoutsias, J. G.en
dc.contributor.authorSakarellos-Daitsiotis, M.en
dc.contributor.authorDetsikas, E.en
dc.contributor.authorTzioufas, A. G.en
dc.contributor.authorSakarellos, C.en
dc.contributor.authorMoutsopoulos, H. M.en
dc.date.accessioned2015-11-24T16:50:34Z-
dc.date.available2015-11-24T16:50:34Z-
dc.identifier.issn0009-9104-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/9621-
dc.rightsDefault Licence-
dc.subjectvesicular stomatitis virusen
dc.subjectautoimmune rheumatic diseasesen
dc.subjectsynthetic peptidesen
dc.subjectro/ssaen
dc.subjectsystemic lupus erythematosusen
dc.subjectsystemic lupus-erythematosusen
dc.subjectro ribonucleoproteinen
dc.subjectsjogrens-syndromeen
dc.subjectrevised criteriaen
dc.subjectimmunoglobulin-gen
dc.subjectproteinsen
dc.subjectclassificationen
dc.subjectautoantibodiesen
dc.subjectheterogeneityen
dc.subjectepitopesen
dc.titleAntibodies to Eyrkk Vesicular Stomatitis Virus-Related Peptide Account Only for a Minority of Anti-Ro60kd Antibodiesen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.secondary<Go to ISI>://A1994PW37700011-
heal.identifier.secondaryhttp://onlinelibrary.wiley.com/store/10.1111/j.1365-2249.1994.tb05506.x/asset/j.1365-2249.1994.tb05506.x.pdf?v=1&t=h0e0r6w2&s=dcb8cb726ad742b46f415dff3bfd39ec73e40d4e-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.publicationDate1994-
heal.abstractPrevious studies demonstrated a possible antigenic relation between the carboxyl terminal portion of anti-Ro60kD autoantigen and a nucleocapsid protein (N) of vesicular stomatitis virus (VSV). In order to investigate whether anti-Ro60kD autoantibodies react with the VSV homologous region of the Ro60kD protein we synthesized, according to Merrifield's method, the EYRKKMDI octapeptide (8p) sharing a common sequence with the N protein of VSV. Sera from 61 patients with autoimmune rheumatic diseases (34 systemic lupus erythematosus (SLE), 21 Sjogren's syndrome (SS) and six rheumatoid arthritis (RA)) as well as 59 from normal blood donors were tested for the presence of anti-Ro60kD autoantibodies by ELISA and immunoblot (IB) and anti-8p antibodies by ELISA. Antibodies to 8p were found in 9/31 of anti-Ro60kD IB-positive sera, 5/30 of anti-Ro60kD-negative sera and 2/59 of normal control sera. The concordance between the anti-8p ELISA and the anti-Ro60kD IB was very poor (chi(2) = 0.71, P = 0.4) in contrast to the anti-Ro60kD ELISA and the anti-Ro IB (chi(2) = 27.6, P = 10(-7)). Subsequent affinity purification of the anti-8p antibodies from a strong positive anti-8p and anti-Ro60kD SLE serum yielded 95% depletion of the anti-8p activity and 37% reduction of the anti-Ro60kD activity. Inhibition assays with the affinity-purified anti-8p antibodies demonstrated that the octapeptide gave 94.5% inhibition of the anti-Ro60kD activity, while Ro60kD protein led to 42.3% inhibition of the anti-8p. Preincubation of the serum with the octapeptide produced 4% inhibition of anti-Ro60kD ELISA. These results indicate that the anti-8p antibodies account only for a minority of the anti-Ro60kD autoantibodies.en
heal.publisherBlackwellen
heal.journalNameClin Exp Immunolen
heal.journalTypepeer reviewed-
heal.fullTextAvailabilityTRUE-
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