Analytical procedure for the in-vial derivatization- extraction of phenolic acids and flavonoids in methanolic and aqueous plant extracts followed by gas chromatography with mass-selective detection (Journal article)

Fiamegos, Y. C./ Nanos, C. G./ Vervoort, J./ Stalikas, C. D.


An in-vial simple method for the combined derivatization and extraction of phenolic acids and flavonoids from plant extracts and their direct determination with GC-MS, is described. The method is taking advantage of the beneficial potentials of phase transfer catalysis (PTC). Catalysts in soluble and polymer-bound form were tested with the latter being the format of choice due to its high reaction yield and facile separation from the rest of the reaction system. Optimization of experimental conditions was established. Chromatographic separation of eight phenolic acids and four flavonoids methylated via the PTC derivatization step was achieved in 45 min. The detection limits for the described GC-MS(SIM) method of analysis ranged between 2 and 40ng/ml whereas limits of quantitation fall in the range 5-118 ng/ml, with flavonoids accounting for the lowest sensitivity due to their multiple reaction behavior. Four methanolic extracts from Tilia europea, Urtica dioica, Mentha spicata and Hypericumperforatum grown wild in north-westem Greece and four aquatic infusions from commercially available Mentha spicata, Origanum dictamnus, Rosemarinus officinalis and Sideritis cretica were analyzed. Good trueness of the method was demonstrated as no matrix effects were found for the analytes concerned. (C) 2004 Elsevier B.V. All rights reserved.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: derivatization,gc,phase transfer catalysis,phenolic acids,flavonoids,performance liquid-chromatography,solid-phase extraction,antioxidant activity,caffeic acid,diode-array,identification,spectrometry,hplc,l.,validation
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/9544
ISSN: 0021-9673
Link: <Go to ISI>://000222284700002
http://ac.els-cdn.com/S0021967304006417/1-s2.0-S0021967304006417-main.pdf?_tid=01c2aa2a183ae04109e898ecba139351&acdnat=1333040744_251a98a4edac624272aba911c9c7b653
Publisher: Elsevier
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

Files in This Item:
File Description SizeFormat 
Fiamegos-2004-Analytical procedure.pdf107.9 kBAdobe PDFView/Open    Request a copy



 Please use this identifier to cite or link to this item:
http://olympias.lib.uoi.gr/jspui/handle/123456789/9544
  This item is a favorite for 0 people.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.