Study of the electrochemical behavior of Disperse Blue 1-modified graphite electrodes. Application to the flow determination of NADH (Journal article)

Stergiou, D. V./ Prodromidis, M. I./ Veltsistas, P. G./ Evmiridis, N. P.

The preparation and the electrochemical study of Disperse Blue 1-chemically modified electrodes (DB1-CME), as well as their efficiency for the electrocatalytic oxidation of NADH is described. The proposed mediator was immobilized by physical adsorption onto graphite electrodes. The electrochemical behavior of DB1-CME was studied with cyclic voltammetry. The electrochemical redox reaction of DB1 was found to be reversible, revealing two well-shaped pair of peaks with formal potentials 152 and -42 mV, respectively, (vs. Ag/AgCl/3M KCl) at pH 6.5. The current I-P has a linear relationship with the scan rate up to 800 mV s(-1), which is indicative for a fast electron transfer kinetics. The dissociation constants of the immobilized DB1 redox couple were calculated pK(1) = 4 and pK(2) = 5. The electrochemical rate constants of the immobilized DB1 were calculated k(1)degrees = 18s(-1) and k(2)degrees = 23s(-1) (Gamma = 2.36 nmol cm(-2)). The modified electrodes were mounted in a flow injection manifold, poised at + 150 mV (vs. Ag/AgCl/3M KCl) and a catalytic current due to the oxidation of NADH was measured. The reproducibility was 1.4% RSD (n = 11 for 30 muM NADH) The behavior of the sensor towards different reducing compounds was investigated. The sensor exhibited good operational and storage stability.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: disperse blue 1-modified graphite electrode,cyclic voltammetry,electrocatalysis of nadh,flow injection analysis,dihydronicotinamide adenine-dinucleotide,chemically-modified electrodes,potential sweep voltammogram,electrocatalytic oxidation,general expression,nile blue,2,6-dichlorophenolindophenol,stability
URI: https://olympias.lib.uoi.gr/jspui/handle/123456789/9342
ISSN: 1040-0397
Link: <Go to ISI>://000221992100011
http://onlinelibrary.wiley.com/store/10.1002/elan.200302902/asset/949_ftp.pdf?v=1&t=h1gmuys2&s=522ed2ef77dff1ec2eaadb6f125723e32125d1dd
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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