Solution structure of an SRYD-containing sequence (250-257) of the fibronectin-like Leishmania gp63 protein by restrained molecular dynamics (Journal article)

Orlewski, P./ Tsikaris, V./ Sakarellos-Daitsiotis, M./ Sakarellos, C./ Soteriadou, K. P./ Marraud, M./ Cung, M. T.

The IASRYDQL synthetic octapeptide (250-257) of the Leishmania major surface glycoprotein gp63 efficiently inhibits parasite attachment to the macrophage receptors in in vitro experiments, and the SRYD-containing tetrapeptide mimics antigenically and functionally the RGDS sequence of fibronectin. The conformational properties of the octapeptide were investigated in dimethylsulfoxide (DMSO) with the combined use of NMR data (vicinal coupling constants, nuclear Overhauser effects (NOEs) and temperature coefficient values), molecular modeling by energy minimization and molecular dynamics. The structure is characterized by the high occurrence, exceeding 95%, of the Arg-Asp side-chain-side-chain ionic interaction, which plays a key role in the backbone folding through a distorted type-I beta-turn involving the Gln(256)-NH to Arg(253)-CO hydrogen bond.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: leishmania gp63,2d nmr,molecular dynamics,dmso solvent box,arginine ionic bridge,beta i-turn,dimethyl-sulfoxide,recognition site,cell-adhesion,rgd,peptides,grgdsp
URI: https://olympias.lib.uoi.gr/jspui/handle/123456789/9203
ISSN: 0929-5666
Link: <Go to ISI>://A1996WA48200007
http://www.springerlink.com/content/h5x231333042l588/fulltext.pdf
Publisher: Kluwer Academic Publishers
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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