Proteome Analysis of Bronchoalveolar Lavage in Individuals from Metsovo, Nonoccupationally Exposed to Asbestos (Journal article)

Archimandriti, D. T./ Dalavanga, Y. A./ Cianti, R./ Bianchi, L./ Manda-Stachouli, C./ Armini, A./ Koukkou, A. I. I./ Rottoli, P./ Constantopoulos, S. H./ Bini, L.


Inhabitants of Metsovo, NW Greece, have been exposed to an asbestos whitewash, resulting in malignant pleural mesothelioma (MPM) and pleural calcifications (PCs). Interestingly, those with PCs (PC(+)) are less prone to MPM. They also have lymphocytic alveolitis, and differences in bronchoalveolar lavage (BAL) proteins, compared with those without pleural calcifications (PC(-)). This may mean a different response to the fiber leading to different susceptibility to neoplasia. To further evaluate this, a proteomic analysis of BAL proteins was performed. Proteomic analysis (2D-electrophoresis/Mass Spectrometry) of BAL in Metsovites nonoccupationally exposed to asbestos revealed increased albumin fragments, alpha 1-antitrypsin, S100-A9 and HSP27, suggesting ongoing inflammation. In those without pleural calcifications, increased expression of acid ceramidase, glutathione-S-transferase and presence of calcyphosin, all involved in cell cycle regulation and death as well as in the detoxification of mutagenic and toxic agents, lend further support to our thesis of possible "protection against neoplasia" in Metsovites with pleural calcifications.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: metsovo lung,asbestos,tremolite,malignant mesothelioma,pleural calcifications,bronchoalveolar lavage,proteomics,two-dimensional electrophoresis,acid ceramidase,glutathione-s-transferase,malignant pleural mesothelioma,bovine serum-albumin,free-radicals,acid ceramidase,lung-cancer,cell-death,2-dimensional electrophoresis,hsp27 oligomerization,ca2+-binding protein
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/8971
ISSN: 1535-3893
Link: <Go to ISI>://000263193300046
http://pubs.acs.org/doi/abs/10.1021/pr800370n
Publisher: American Chemical Society
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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