alpha- and beta-Aspartyl peptide ester formation via aspartimide ring opening (Journal article)

Stathopoulos, P./ Papas, S./ Kostidis, S./ Tsikaris, V.

The undesirable reaction of aspartimide formation has been proved to occur under both acid and base conditions in solid-phase peptide synthesis and is dependent on the P-carboxyl protecting group, the acid or base used during the synthesis, as well as the peptide sequence. The hydrolysis of aspartimide-containing peptides, especially during HPLC purification, yields a mixture of alpha- and beta-aspartyl peptides that can not be purified easily. A previous study demonstrated that treatment of aspartimide-containing peptides With methanol in the presence of 2% diisopropylethylamine in solution leads to a- and P-aspartyl peptide methyl esters. Taking advantage of these results and aiming at elucidating the optimal conditions for aspartimide ring opening, the effect of different types and concentrations of alcohols (primary and secondary) and bases (diisopropylethylamine, collidine, 4-pyrrolidinopyridine, 1-methyl-2-pyrrolidone, piperidine and KCN) was tested at various temperatures and reaction times. The best results were obtained with a combination of a primary alcohol and diisopropylethylamine, while aspartimide ring opening by secondary alcohols occurred only at high temperatures. The optimal conditions were also applied to solid-phase peptide synthesis. Copyright (c) 2005 European Peptide Society and John Wiley & Sons, Ltd.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: aspartimide,aspartimide chemistry,alpha- and beta-aspartyl peptide esters,fmoc-based spps,aminosuccinyl peptides,product formation,sequences
URI: https://olympias.lib.uoi.gr/jspui/handle/123456789/8943
ISSN: 1075-2617
Link: <Go to ISI>://000232814800008
http://onlinelibrary.wiley.com/store/10.1002/psc.675/asset/675_ftp.pdf?v=1&t=h0f8smfx&s=5575ff75dd55ae0f7d53659d14165652bc6f6095
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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