Ozone Monitoring Based on a Biosensor Concept Utilizing a Reagentless Alcohol Oxidase Electrode (Journal article)

D.V. Stergiou,/ M.I. Prodromidis,/ P.G. Veltsistas,/ N. Evmiridis

An electrochemical method based on the concept of a biosensor for the monitoring of ozone is described for first time. The proposed method includes two parts:"‰ a selective sorbent for ozone, that is, eugenol, and a formaldehyde amperometric biosensor mounted into a flow-through cell. Ozone adds rapidly to the double bond of the allyl group of eugenol, which has been immobilized onto a hydrophobic C-18 reactor and the so produced formaldehyde is collected into the working buffer solution (sampler) and pumped to the detector. A multimembrane assembly consisting of an alcohol oxidase-modified nylon membrane sandwiched between an outer polycarbonate and an inner cellulose acetate membrane was fitted onto a Pt electrode and the enzymatically produced H2O2 was monitored at +0.65 V (vs Ag/AgCl/KCl 3 M). Under optimum conditions, a linear calibration curve over the concentration range 3β�’200 ΞΌgΒ·mL-1 ozone was constructed. The detection limit (S/N = 3) was calculated at 1.1 ΞΌgΒ·mL-1 ozone. The proposed method is interference-free from other gases such as O2, Ar, N2, N2O, NOCl, SO2, NH3, and CO2, which were tested at concentrations >200-fold higher than that of 100 ΞΌgΒ·mL-1 ozone used for comparison. Besides selectivity, the method is easy to perform and reproducible; its applicability in synthetic gaseous samples is also demonstrated.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
URI: https://olympias.lib.uoi.gr/jspui/handle/123456789/8737
Link: http://pubs.acs.org/doi/abs/10.1021/ac060230h
Publisher: American Chemical Society
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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