Acid-induced phase separation of anionic surfactants for the extraction of 1,4-dichlorobenzene from honey prior to liquid chromatography (Journal article)

Paleologos, E. K./ Giannakopoulos, S. S./ Zygoura, P. D./ Kontominas, M. G.

The acid-induced liquid-liquid phase separation of anionic surfactants in aqueous solutions and its applicability to cloud point extraction methodology were applied as a tool for the extraction of 1,4-dichlorobenzene (p-DCB) from aqueous samples. p-DCB is extracted into the micelles of sodium dodecane sulfonate (SDSA) in a 4.2 M HCl solution. The micellar phase is separated from the bulk aqueous solution after centrifugation and collected from the surface of the suspension. The micellar extracts are injected into a high-performance liquid chromatographic apparatus and quantified at 225 nm with a reference wavelength of 280 nm. Following the proposed methodology, a preconcentration factor of ca. 160 is achieved (starting from 50 mL solutions) allowing for detection limits at the low Ag/L level. Application to honey samples produced detection limits of 2.5 Ag/kg with quantification limits of 7.5 Ag/kg, while the recoveries of the method ranged from 85% at high concentrations to 95% at lower concentrations of p-DCB. The combined uncertainty of the entire analytical procedure was 4.5% at the concentration level of 30 Ag/kg allowing for reliable and reproducible results for the determination of p-DCB at the concentration levels considered as thresholds for EU and U. S. legislation (10 Ag/kg).
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: 1,4-dichlorobenzene,anionic surfactants,phase separation,honey,hplc-uv,volatile organic-compounds,assisted back-extraction,gas-chromatography,aromatic-hydrocarbons,mass spectrometry,preconcentration,microwave,samples
ISSN: 0021-8561
Link: <Go to ISI>://000239120200005
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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