Isolation, chemical characterization, and subcellular distribution of 1-O-alkyl-2-acetyl-sn-glycerol in Tetrahymena pyriformis cells (Journal article)

Tellis, C./ Tsoukatos, D. C./ Lekka, M. E.


1-O-Hexadecyl-2-acetyl-sn-glycerol, the immediate precursor of platelet-activating factor (PAF) in its de nova formation, was detected in the protozoon Tetrahymena pyriformis. It was purified from the total lipid extract by TLC, after successive developments in two different solvent systems. Characterization was assessed by (a) gas-liquid chromatography with electron capture detection, and (b) gas chromatography combined with mass spectrometry in selected ion monitoring mode, after derivatization with heptafluorob utyric acid anhydride and tert-butyldimethylchlorosilane/imidazole respectively. Its quantity was found to be 0.1 nmol/10(7) cells from the CC-MS, using authentic alkylacetylglycerol as external standard, Cell fractionation revealed that alkylacetylglycerol is located exclusively in the microsomal fraction of the protozoon. Previously, we have reported the occurrence of PAF in the microsomal fraction, as well as a dithiothreitol-insensitive CDP-choline: cholinephosphotransferase activity that utilizes exogenous alkylacetylglycerol as substrate in the mitochondrial and microsomal fractions. The above findings indicate that PAF can be formed in the cell by the de novo pathway.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: alkylacetylglycerol,diacylglycerol,gc-ms,platelet-activating factor,tetrahymena pyriformis,platelet-activating-factor,denovo biosynthesis,rabbit platelets,hl-60 cells,paf,1-alkyl-2-acetyl-sn-glycerols,glycerophospholipids,conversion,calcium
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/8305
ISSN: 0021-924X
Link: <Go to ISI>://A1996UE81600033
Publisher: Oxford University Press
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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