Ion chromatographic method for the simultaneous determination of nitrite and nitrate by post-column indirect fluorescence detection (Journal article)

Stalikas, C. D./ Konidari, C. N./ Nanos, C. G.


This short paper highlights the suitability of ion chromatography with post-column indirect fluorescence detection to determine simultaneously nitrite and nitrate based on the quenching of tryptophan native fluorescence. The method uses an enhanced fluorescence mobile phase containing tryptophan and detects the suppression of fluorescence of the mobile phase due to the elution of the target ions. The phenomenon of fluorescence quenching of tryptophan is highly induced by the presence of phosphate ions. The quenched fluorescence intensity exhibits concentration dependence in the range 1-25 mg/l and 3-65 mg/l for nitrite and nitrate, respectively. The relative standard deviation for five replicates of a standard solution containing a mixture of 5 mg/l of nitrite and 10 mg/l of nitrate lies around 2.8%. This simple coupling technique results in a relatively sensitive. fast. and accurate method, allowing for both qualitative and quantitative analysis of nitrite and nitrate. The method can easily be implemented to real samples such as foodstuffs, fertilizers and soils and is proven to be precise and accurate when compared with reference methods. (C) 2003 Elsevier Science B.V. All rights reserved.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: indirect detection,detection, lc,food analysis,soil,fertilizers,environmental analysis,nitrite,nitrate,inorganic anions,flow-injection analysis,spectrophotometric determination,water samples
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/8292
ISSN: 0021-9673
Link: <Go to ISI>://000183799200021
http://ac.els-cdn.com/S0021967303007350/1-s2.0-S0021967303007350-main.pdf?_tid=03a4c9831694b00a6d7b3142ae23bf18&acdnat=1333030794_8bb93edf2688fb21f27d7f0bb502cf39
Publisher: Elsevier
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

Files in This Item:
File Description SizeFormat 
Stalikas-2003-Ion chromatographic.pdf125.14 kBAdobe PDFView/Open    Request a copy



 Please use this identifier to cite or link to this item:
http://olympias.lib.uoi.gr/jspui/handle/123456789/8292
  This item is a favorite for 0 people.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.