A three-residue cyclic scaffold of non-RGD containing peptide analogues as platelet aggregation inhibitors: Design, synthesis, and structure-function relationships (Journal article)

Stavrakoudis, A./ Bizos, G./ Eleftheriadis, D./ Kouki, A./ Panou-Pomonis, E./ Sakarellos-Daitsiotis, M./ Sakarellos, C./ Tsoukatos, D./ Tsikaris, V.

Antagonists of fibrinogen at the GPIIb/IIIa receptor, which is the most abundant membrane protein on the platelet surface, are tinder active investigation as potential antithrombotics. The critical interaction between GPIIb/IIIa and fibrinogen can be inhibited by either linear or cyclic RGDS-containing peptides, which have been proved as lead compounds in the design of platelet aggregation inhibitors. In this study we present the design and construction of it new class of cyclic (S,S) non-RGD containing peptide sequences, using two Cys cis a structural scaffold for the development of antiaggregatory agents. The (S,S)-CDC- sequence was incorporated as a conformational constraint, in molecules bearing at least one positive charge with the general formula (S,S)XCDCZ, where X = Ac-Arg, Pro-Arg, Pro-Ser-Lys, and Pro-Ser-Arg, and Z = -NH2 and Arg-NH2. Investigation of the structure-function relationships was performed on the basis of (a) the local conformation induced by the (S,S)-CDC motif (b) the distance of the positively (R-C-zeta or K-N-zeta) and negatively (D-C-gamma) charged centers, (c) the presence of a second positive or negative charge on the molecule, and (d) the orientation of the basic and acidic side chains defined by, the pseudo dihedral angle (Pdo), which is formed by, the R-C-zeta, R-C-alpha, D-C-alpha, and D-C-gamma atoms in the case of (S,S)-RCDC and by the K-A K-C-alpha, D-C-alpha, and D-C-gamma atoms in the case of (S,S)-KCDC (C) 2001 John Wiley & Sons, Inc.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: cyclic non-rgd containing analogues,cystine non-rgd analogues,platelet aggregation inhibitors,cyclization on solid support,disulfide bond formation,arg-gly-asp,fibrinogen receptor antagonist,alpha(v)beta(3) antagonists,integrin alpha(iib)beta(3),secondary structure,highly potent,recognition,protein,sequence,program
URI: https://olympias.lib.uoi.gr/jspui/handle/123456789/8288
ISSN: 0006-3525
Link: <Go to ISI>://000171763200004
Publisher: Wiley
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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