Intermediates of thiamine catalysis immobilized on silica surface as active biocatalysts for alpha-ketoacid decarboxylation (Journal article)

Stamatis, A./ Malandrinos, G./ Butler, I. S./ Hadjiliadis, N./ Louloudi, M.

Thiamine-dependent enzymes catalyse the decarboxylation of alpha-ketoacids, by both non-oxidative and oxidative mechanisms. Based on the ability of thiamine-cofactor to catalyse itself the decarboxylation of pyruvate to some extent, we have immobilized on a silica surface two 'active aldehyde' intermediates of thiamine catalysis, 2-alpha-hydroxybenzyl-thiamine pyrophosphate (HBTPP) and 2-alpha-hydroxyethyl-thiamine pyrophosphate (HETPP). The two intermediates have been tethered by a convenient method on silica support via their phosphate groups providing the covalently heterogenised biomolecules [HBTh-OP2O6-SiO3/2](n)center dot xSiO(2), and [HETh-OP2O6-SiO3/2](n)center dot xSiO(2). These bio-composite materials have been evaluated as catalysts for pyruvate and benzoyl-formate decarboxylation in either the presence or not of an aldehyde additive. Our data show that they are stable, very effective and recyclable catalysts for the production of 2-hydroxy-ketones, acetoine and benzoin. Their catalytic behavior is much better than the corresponding behavior of the homogeneous thiamine-systems due to the selected immobilization mode which bears similarities to that of the thiamine-cofactor binding to the protein. Considering our results, possible catalytic pathways of the prepared bio-composite materials are suggested. (C) 2006 Elsevier B.V. All rights reserved.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: thiamine catalysis,decarboxylation,immobilised biocatalysts,modified silica,2-hydroxy-ketones,diphosphate-dependent enzymes,sol-gel materials,benzoylformate decarboxylase,crystal-structure,metal-complexes,pyrophosphate,mechanism,models,cadmium(ii),resolution
URI: https://olympias.lib.uoi.gr/jspui/handle/123456789/8270
ISSN: 1381-1169
Link: <Go to ISI>://000245880900017
http://ac.els-cdn.com/S1381116906014075/1-s2.0-S1381116906014075-main.pdf?_tid=7c1b87479e8b7a862c552f056d00b985&acdnat=1333034662_fa5d43f85f387379c81307d9bc4dc3e0
Publisher: Elsevier
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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