Gas chromatographic determination of carbonyl compounds in biological and oil samples by headspace single-drop microextraction with in-drop derivatisation (Journal article)

Fiamegos, Y. C./ Stalikas, C. D.


A suitable method for the gas chromatographic determination of 10 characteristic carbonyls in biological and oil samples based on the in-drop formation of hydrazones by using 2,4,6-trichlorophenylhydrazine (TCPH), has been developed. The derivatisation-extraction procedure was optimized separately for aqueous and oil samples with respect to the appropriate organic drop solvent, drop volume, in-drop TCPH concentration, sample stirring rate, temperature during single-drop microextraction (SDME), reaction time and headspace-to-sample volume ratio. The optimization showed differentiation of optimum values between the studied matrices. The limits of detection were found to range from 0.001 to 0.003 mu g mL(-1) for the aqueous biological samples and from 0.06 to 0.20 mu g mL(-1) for the oil samples. The limits of quantification were in the range of 0.003-0.010 mu g mL(-1) and 0.020-0.059 mu g mL(-1) for aqueous and oil samples, respectively The overall relative standard deviations of the within-day repeatability and between-day reproducibility were < 4.4% and < 8.2% for the aqueous biological samples and < 3.9% and < 7.4% for the oxidized oil samples. (c) 2008 Elsevier B.V. All rights reserved.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: headspace single-drop,microextraction 2,4,6-trichlorophenylhydrazine,aldehydes oil,plasma urine,solid-phase microextraction,on-fiber derivatization,electron-capture detection,mass-selective detection,lipid-peroxidation,human blood,thermal-desorption,fatty-acids,aldehydes,spectrometry
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/8032
ISSN: 0003-2670
Link: <Go to ISI>://000253616500005
http://ac.els-cdn.com/S0003267008000299/1-s2.0-S0003267008000299-main.pdf?_tid=bc649cd6541b25d91d3f8d6c13086de9&acdnat=1333040770_a324c5491e366865b8d2df111caf3e78
Publisher: Elsevier
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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