Catalytic behavior of Pseudomonas cepacia lipase in w/o microemulsions (Journal article)

Stamatis, H./ Xenakis, A./ Dimitriadis, E./ Kolisis, F. N.


The activity of purified Pseudomonas cepacia lipase has been investigated in esterification reactions of various aliphatic alcohols with natural fatty acids. The reactions were carried out in microemulsions formed in isooctane by bis-(2-ethylhexyl)sulfosuccinate sodium salt (AOT). Kinetic studies showed that the reaction follows a ping-pong bi-bi mechanism with inhibition by both substrates. The apparent kinetic parameters of the reaction were found to be K(m octanol) = 310 mM, K(m lauric acid) = 78 mM, and V(max) = 250 mumol min(-1) mg(-1). The same system was used for the synthesis of mono- and diglycerides from glycerol and lauric acid, which was successful at very low w(o) values. The catalytic behavior of P. cepacia lipase was also studied in esterification reactions performed in a nonionic microemulsion system formulated by tetraethyleneglycoldodecylether (C(12)E(4)). The optimum activity was found at about w(o) = 8. The apparent values of V(max app) and K(m app) for octanol were calculated and found to be 100 mumol min(-1) mg(-1) and 76 mM, respectively. (c) 1995 John Wiley & Sons, Inc.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/7922
ISSN: 0006-3592
Link: http://www.ncbi.nlm.nih.gov/pubmed/18623049
http://onlinelibrary.wiley.com/store/10.1002/bit.260450106/asset/260450106_ftp.pdf?v=1&t=h1j04et1&s=278c62762d5d086d983083391ddca71da983058f
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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