An Open Reading Frame in the Approximately 28-Kb Tox-Argk Gene-Cluster Encodes a Polypeptide with Homology to Fatty-Acid Desaturases (Journal article)

Hatziloukas, E./ Panopoulos, N. J./ Delis, S./ Prosen, D. E./ Schaad, N. W.


Part of an apparent open reading frame in the tox-argK gene cluster of Pseudomonas syringae pathovar phaseolicola (Psp) potentially encodes a polypeptide with sequence similarity to fatty acid desaturases (DES). Escherichia coli B expressing this segment under T7 promoter control produced a 34-kDa polypeptide. The possible involvement of a DES in facilitating phaseolotoxin (Ptx) secretion at the low temperatures normally required for its synthesis and the evolutionary implications about the origin of the tox-argK gene cluster are discussed.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών
Keywords: phaseolotoxin,bean halo blight,ornithine carbamoyltransferase,pseudomonas syringae,syringae pv phaseolicola,bacteriophage-t7 rna-polymerase,pseudomonas-syringae,ornithine carbamoyltransferase,copy-number,phaseolotoxin,DNA,expression,cyanobac
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/7911
ISSN: 0378-1119
Link: <Go to ISI>://A1995TK18700011
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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