Smectite clays as solid supports for immobilization of beta-glucosidase: Synthesis, characterization, and biochemical properties (Journal article)

Serefoglou, E./ Litina, K./ Gournis, D./ Kalogeris, E./ Tzialla, A. A./ Pavlidis, I. V./ Stamatis, H./ Maccallini, E./ Lubomska, M./ Rudolf, P.

Nanomaterials as solid supports can improve the efficiency of immobilized enzymes by reducing diffusional limitation as well as by increasing the surface area per mass unit and therefore improving enzyme loading. In this work, beta-glucosidase from almonds was immobilized on two smectite nanoclays. The resulting hybrid biocatalysts were characterized by a combination of powder X-ray diffraction, X-ray photoelectron spectroscopy, thermogravimetric. analysis, differential thermal analysis, and infrared spectroscopy. Biochemical studies showed an improved thermostability of the immobilized enzyme as well as enhanced performance at higher temperatures and in a wider pH range.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών
Keywords: ray photoelectron-spectroscopy,pyrococcus-furiosus,crystal-structure,enzyme-activity,montmorillonite,stability,adsorption,biosensor,oxidase,binding
ISSN: 0897-4756
Link: <Go to ISI>://000256854800045
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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