Heterologous expression of a hyperthermophilic alpha-amylase in xanthan gum producing Xanthomonas campestris cells (Journal article)

Konsoula, Z./ Liakopoulou-Kyriakides, M./ Perysinakis, A./ Chira, P./ Afendra, A./ Drainas, C./ Kyriakidis, D. A.

A hyperthermophilic alpha-amylase encoding gene from Pyrococcus woesei was transferred and expressed in Xanthomonas campestris ATCC 13951. The heterologous alpha-amylase activity was detected in the intracellular fraction of X. campestris and presented similar thermostability and catalytic properties with the native P. woesei enzyme. The recombinant alpha-amylase was found to be stable at 90C for 4 h and within the same period it retained more than 50% of its initial activity at 110C. Furthermore, X. campestris transformants produced similar levels of recombinant alpha-amylase activity regardless of the carbon source present in the growth medium, whereas the native X. campestris alpha-amylase production was highly dependent on starch availability and it was suppressed in the presence of glucose or other reducing sugars. On the other hand, xanthan gum yield, which appeared to be similar for both wild type and recombinant X. campestris strains, was enhanced at higher starch or glucose concentrations. Evidence presented in this study supports that X. campestris is a promising cell factory for the co-production of recombinant hyperthermophilic alpha-amylase and xanthan gum.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών
Keywords: hyperthermophilicity,alpha-amylase,xanthan gum,xanthomonas campestris,pyrococcus-woesei,cloning,sequence,extract,growth,gene
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/7745
ISSN: 0273-2289
Link: <Go to ISI>://000254847500001
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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