Neurotransmitter phenotype-specific expression changes in developing sympathetic neurons (Journal article)
Apostolova, G./ Dorn, R./ Ka, S./ Hallbook, F./ Lundeberg, J./ Liser, K./ Hakim, V./ Brodski, C./ Michaelidis, T. M./ Dechant, G.
During late developmental phases individual sympathetic neurons undergo a switch from noradrenergic to cholinergic neurotransmission. This phenomenon of plasticity depends on target-derived signals in vivo and is triggered by neurotrophic factors in neuronal cultures. To analyze genome-wide expression differences between the two transmitter phenotypes we employed DNA microarrays. RNA expression profiles were obtained from chick paravertebral sympathetic ganglia, treated with neurotrophin 3, glial cell line-derived neurotrophic factor or ciliary neurotrophic factor, all of which stimulate cholinergic differentiation. Results were compared with the effect of nerve growth factor, which functions as a pro-noradrenergic stimulus. The gene set common to all three comparisons defined the noradrenergic and cholinergic synexpression groups. Several functional categories, such as signal transduction, G-protein-coupled signaling, cation transport, neurogenesis and synaptic transmission, were enriched in these groups. Experiments based on the prediction that some of the identified genes play a role in the neurotransmitter switch identified bone morphogenetic protein signaling as an inhibitor of cholinergic differentiation. (c) 2007 Elsevier Inc. All rights reserved.
|Institution and School/Department of submitter:||Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών|
|Keywords:||acetylcholine,noradrenalin,sympathetic,neurotrophic,microarray,nerve growth-factor,cholinergic differentiation,neural crest,in-vivo,ganglia,system,induction,cytokines,pathways,signals|
|Link:||<Go to ISI>://000248057600001|
|Appears in Collections:||Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)|
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