Lipase biocatalytic processes in surfactant free microemulsion-like ternary systems and related organogels (Journal article)

Zoumpanioti, M./ Karali, M./ Xenakis, A./ Stamatis, H.


Lipases from Rhizomucor miehei (RmL) and Candida antartica (CaL-B) entrapped in surfactant free microemulsion-like ternary systems consisting of n-hexane, short chain alcohols (1-propanol or 2-methyl-2-propanol) and water, keep their catalytic activity at 30 degrees C, catalyzing efficiently the esterification of fatty acids or natural phenolic acids including cinnamic acid derivatives. High conversions (up to 95%) were obtained with both lipases entrapped in various ternary systems. Lipases catalytic efficiency and stability was influenced by the composition of the ternary systems used. Hydroxypropylmethyl cellulose (HPMC) microemulsions-based organogels (MBGs) formulated with various surfactant free microemulsion-like systems were used as a matrix for enzyme immobilization. These lipase-containing MBGs prove to be a novel solid-phase catalyst for use, under mild conditions, in non-polar solvents as well as in solvent free systems. CaL-B immobilized in surfactant free MBGs keep its catalytic activity for a storage period of 8 months, while the corresponding residual activity of immobilized RmL was decreased by 50%. The stability of enzymes in such surfactant free MBGs is higher than that observed in MBGs formulated with surfactant based w/o microemulsions. Lipases-containing surfactant free MBGs were used in seven consecutive batch syntheses of fatty esters with only 10-15% loss of activity. (c) 2005 Elsevier Inc. All rights reserved.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών
Keywords: surfactant free microemulsions,organogels,lipase,esterification,immobilization,stability,phenolic acids,organic-solvent mixtures,in-oil microemulsions,detergentless microemulsions,enzymatic-reactions,feruloyl esterase,bioorganic reactions,p
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/7709
ISSN: 0141-0229
Link: <Go to ISI>://000238932500002
http://ac.els-cdn.com/S0141022905005387/1-s2.0-S0141022905005387-main.pdf?_tid=50302348cad49162a6bacb936ce97be7&acdnat=1335510833_6cf6f3507d90def9fe1e313625052dbd
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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