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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Tooley, P. W. | en |
| dc.contributor.author | Hatziloukas, E. | en |
| dc.contributor.author | Scott, D. L. | en |
| dc.contributor.author | Carras, M. M. | en |
| dc.date.accessioned | 2015-11-24T16:32:50Z | - |
| dc.date.available | 2015-11-24T16:32:50Z | - |
| dc.identifier.issn | 0706-0661 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/7593 | - |
| dc.rights | Default Licence | - |
| dc.subject | biotin | en |
| dc.subject | digoxigenin | en |
| dc.subject | paramagnetic | en |
| dc.subject | colorimetric | en |
| dc.subject | molecular biology | en |
| dc.subject | potato late blight | en |
| dc.subject | ribosomal DNA | en |
| dc.subject | reaction assay | en |
| dc.subject | chlamydia-trachomatis | en |
| dc.subject | mitochondrial-DNA | en |
| dc.subject | sequence-analysis | en |
| dc.subject | infected potato | en |
| dc.subject | pcr technique | en |
| dc.subject | linkage map | en |
| dc.subject | identification | en |
| dc.title | Use of ligase chain reaction for enhanced detection of Phytophthora infestans | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.secondary | <Go to ISI>://000178873800007 | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών | el |
| heal.publicationDate | 2002 | - |
| heal.abstract | Sequences of the internal transcribed spacer region 2 (ITS 2) of ribosomal DNA from 10 different species of Phytophthora were aligned, and regions of dissimilarity were used to construct ligase chain reaction (LCR) primers for detecting the late blight pathogen, Phytophthora infestans. Ligase chain reaction primers allow differentiation of species down to a single base pair difference. The primers have greater specificity than previously described polymerase chain reaction (PCR) primers and allow differentiation of R infestans, Phytophthora mirabilis, and Phytophthora phaseoli, which contain identical ITS sequences, from many other Phytophthora species. Ligase chain reaction was coupled with PCR to increase sensitivity of P. infestans detection and can serve as an enhancement to existing PCR protocols. By labeling LCR primers with biotin and digoxigenin, the LCR was combined with an ELISA-based detection system to improve sensitivity and ease of application. | en |
| heal.journalName | Canadian Journal of Plant Pathology-Revue Canadienne De Phytopathologie | en |
| heal.journalType | peer reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) | |
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