Co-production of ice nuclei and xanthan gum by transformed Xanthomonas campestris grown in sugar beet molasses (Journal article)

Afendra, A. S./ Yiannaki, E. E./ Palaiomylitou, M. A./ Kyriakidis, D. A./ Drainas, C.

Two recombinant plasmids, expressing ice nucleation activity, were constructed and named pCPP30inaZ and pCPP38inaZ. They were transferred to the ice-negative, xanthan-producing Xanthomonas campestris pv. campestris by electroporation. The transformants were used for co-production of xanthan gum and ice nuclei from sugar beet molasses. The highest values obtained were 20 g l(-1) and 10(18) ice nuclei ml(-1), respectively. The above values fulfil the criteria for industrial manipulation. This is the first report on co-formation of two products by a transformed X. campestris strain.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών
Keywords: fermentation,ice nucleation gene,molasses,xanthan gum,xanthomonas campestris,nucleation gene,pseudomonas-syringae,expression,bacteria,strains
ISSN: 0141-5492
Link: <Go to ISI>://000174634300014
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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