Μελέτη του ρολού των μη στεροειδών αντιφλεγμονωδών φάρμακων στην περιφερική νευρική αναγέννηση σε πειραματικό μοντέλο τελικό-πλάγιας νευρικής συρραφής (Doctoral thesis)
Γιαννούλης, Διονύσιος Κ.
Objectives: The main aim of this study was the evaluation of the effect of the Non-Steroid Anti-Inflammatory Drugs in peripheral nerve regeneration in an experimental model of double end-to-side neurorrhaphy in rats. Specifically in this study, we tried to access the positive or negative effect of the NSAIDs ibuprofen and naproxen in nerve regeneration. Materials-Methods: For the completion of the study we used 44 adult male Wistar rats who were divided into 4 groups: Group A where the peroneal nerve was dissected and repaired with a double end-to-side neurorrhaphy to the tibial nerve with the distance between the 2 neurorrhaphies to be 6 mm, Group B in which the same procedures as in Group A were performed with the difference that n this group the NSAID ibuprofen was administered to the rats for 7 days after the operation, Group C in which the same procedures as in group A were performed with the difference that in this group the NSAID naproxen was administered to the rats for 7 days after the operation and Group D in which the same procedures as in group B were performed with the difference that in this group a compression injury to the donor nerve-tibial nerve was done. In all the rats the contralateral limb was used as control. The functional evaluation of the peroneal nerve for nerve regeneration was performed before and after the operation up to the 56th day postoperative with the use f Peroneal Function Index (PFI). The same day a second operation took place and nerve tissue samples for histopathological examination (density of neuraxons per surface unit) and the anterior tibialis muscle was dissected for weight evaluation between the operated and the uninjured side. Results: From the evaluation of the functional and histopathological results the animals of Group B had statistically significant better-superior results compared to the animals of Group A and C. Also Group B had better results in regard to the weight of the anterior tibialis muscle compared to Group A and C. From this study, we report that ibuprofen can stimulate peripheral nerve regeneration in an experimental model of double end-to-side neurorrhaphy in rats. Naproxen does not seem to have the same effect. In Group D due to the donor nerve injury, we could not observe any improvement in the regeneration phase. Conclusion: This study reports that after peripheral nerve injury the use of the NSAID ibuprofen can stimulate peripheral nerve regeneration in an experimental mode of double end-to-side neurorrhapy in rats. This medication can be used to improve the nerve rehabilitation in humans after nerve injury. Further studies to determine the exact dosage and duration of use are needed to extract safer results.
|Institution and School/Department of submitter:||Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής|
|Subject classification:||Νευρικό σύστημα -- Αναγέννηση|
|Keywords:||Περιφερική νευρική αναγέννηση,Μη στεροειδή αντιφλεγμονώδη,Ιβουπροφαίνη,Τελικο-πλάγια νευροσυρραφή,Peripheral nerve regeneration,Νon-steroid anti-inflammatory drugs,Ιbuprofen,Εnd to side neurorrhaphy|
|Appears in Collections:||Διδακτορικές Διατριβές|
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|Δ.Δ. ΓΙΑΝΝΟΥΛΗΣ ΔΙΟΝΥΣΙΟΣ Κ. 2019.pdf||7.27 MB||Adobe PDF||View/Open|
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