Development of nanobiocatalytic systems through on the immobilisation of hydrolases on graphen nanoparticles (Master thesis)

Ορφανάκης, Γεώργιος

This dissertation is about the development of novel protocols for enzyme immobilization on nanomaterials. The enzymes used were of the hydrolases category, specifically β-D-glucosidase and invertase, with the outermost aim being the use of the nanobiocatalytic systems in the conversion of industrial agriculture waste into useful products. The most used enzyme throughout this project was β-D-glucosidase which we managed to immobilize via novel methods that utilize the chemical compound APTES. This led to very stable nanobiocatalytic systems with enhanced level of activity in naturally perceived extreme conditions. The characterization of the materials as well as of the immobilized enzymes was achieved through the use of spectroscopic techniques such as FT-IR, raman and AFM. The knowhow acquired through the immobilization and experimental procedures was then used with success in the immobilization of the enzyme invertase. Finally, we managed to create a nanobiocatalytic system involving both enzymes simultaneously immobilized in many layers with the outer layer belonging to invertase. This final product proved itself extremely handful in hydrolyzing a mix of cellobiose and sucrose, both being industrial wastes. Part of the results has already been published in the international scientific magazine "Frontiers in Materials" under the title "Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of β-Glucosidase: Synthesis, Characterization, and Biocatalytic Properties".
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών
Subject classification: Γραφένιο
Keywords: Γραφένιο,Προηγμένα νανοϋλικά
Appears in Collections:Διατριβές Μεταπτυχιακής Έρευνας (Masters)

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