Study of Cistus biodiversity in Greece and genotypes selection for active chemicals with biotechnological approaches (Doctoral thesis)

Κώστας, Στέφανος Γ.

From 128 genotypes of Cistus creticus, a native species of Greece, plant material was collected aiming at rooting of their cuttings, production of plants for establishing plant collection, genetic classification and determination of produced polyphenols and terpenes. The collection regions were Sisses of Rethymno (29 genotypes), Chalkidiki (28), Akrotiri (11), Manoliopoulo (5), Kandanos (21) and Floria (9) of Chania, Menidi of Aitoloakarnania (2), Analipsi of Ilia (5), Pieria Mountains (1), Volos (2), Tinos (7), Agrinio (4) and Chios (5). The outdoor plant collection was established in the fields of the Laboratory of Floriculture, in the Experimental Farm of the Aristotle University, containing three plants from each genotype for which their morphological traits were recorded with emphasis on flowering. The rooting of cuttings was studied (with the application of K-IBA) on shoots harvested from the plants of the native populations during the four seasons of the year. Then, the genetic analysis followed by using the molecular markers ISSRs. At the same time, chemical analysis of the young leaves of all genotypes was performed to determine the polyphenols using LC-MS/MS, and with GC-MS for terpenes detection. The results showed that the use of the rooting regulator K-IBA at concentrations of 2,000 and 4,000 ppm resulted in the highest rooting rates in Winter and Autumn. For ornamental use of C. creticus, the most suitable population was that of Tinos, as it produced the biggest number of flowers and had a long flowering duration. The use of molecular markers revealed that the populations of Sisses and Chalkidiki (C. creticus subsp. creticus) are not distinct from each other and are genetically close to most of the populations. For the clones of Kandanos, Tinos and Menidi, it is proposed to group them in the sub-species C. creticus subsp. eriocephalus (form much less glandular trichomes on their leaves). Regarding the polyphenols concentrations, it was found that the populations with highest yields were from Sisses, Menidi and Chalkidiki. Correspondingly, the highest values of terpenes were obtained from the population of Chalkidiki. Considering the chemical analyses, in combination with the morphlogical description, it results that for polyphenols production the proposed genotypes are S29, S26, S15 and S28, while for terpenes production the genotypes are C23, C12, C16, C26 and S4.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών
Subject classification: Μόσχευμα
Keywords: Ριζοβολία,Μοσχεύματα,Λαδανιά,Λάδανο,Μοριακοί δείκτες,Πολυφαινόλες,Τερπένια,Rooting,Cuttings,Ladania,Ladano,Molecular markers,Polyphenols,Terpenes
Appears in Collections:Διδακτορικές Διατριβές

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