Cytotoxic effects of novel vanadium complexes in malignant cell lines (Doctoral thesis)

Παπαναστασίου, Γεώργιος Ευθ.


n this dissertation we studied the antitumor properties of two novel vanadium complexes andparticularly the V(V)OC18DEA (Complex 1) and V(IV)Od-tocDPA (Complex 2). The following cell lineswere used: a) leiomyosarcoma Wistar rat cells (LMS), b) human osteosarcoma cells (U2-OS) and c) normal human foetal lung fibroblasts (MRC-5). The ability of cells to proliferate after incubation withincreasing concentrations of the vanadium complexes was measured using the MTT assay.Experiments were also performed to study LMS cells colony formation efficiency following exposure tovanadium complexes and the mechanism of cell death using flow cytometry (apoptosis/necrosis andcell cycle analysis). The results showed that the Complex 1 has potent cytotoxic activity withoutdisplaying selectivity (acting both in cancer and normal cells) while inhibiting the ability of the LMS cellsto form colonies. At higher concentrations the complex induced cell apoptosis and cell cycle arrest byreducing the number of cells in G1 and S phase and increasing them in G2/M phase. The Complex 2had weaker cytotoxic activity than the Complex 1 and failed to inhibit the formation of LMS cellcolonies. The Complex 1 caused cell cycle arrest in G2/M and S phase without induction of cellapoptosis. In conclusion, vanadium complexes (V)OC18DEA and V(IV)Od-tocDPA possess potentcytotoxic activity but further experiments are required to increase their selectivity to kill cancer cells andto determine the precise mechanism of action.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Subject classification: Βανάδιο
Keywords: Βανάδιο,Σύμπλοκα βαναδίου,Οστεοσάρκωμα,Λειομυοσάρκωμα,Καρκίνος,Απόπτωση,Κυτταρική επιβίωση,Κυτταρικός κύκλος,Vanadium complexes,Vanadium,Osteosarcoma,Leiomyosarcoma,Cancer,Apoptosis,Cell viability,Cell cycle
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/29212
Appears in Collections:Διδακτορικές Διατριβές

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