Development of automated computer systems that generate low intensity static radiofrequency electromagnetic fields (Doctoral thesis)

Αυδίκος, Αντώνιος Χρ.


In the present study the effects of low intensity static radiofrequency electromagnetic fields causing no thermal phenomena, on leiomyosarcoma cell lines (LMS) isolated from tumors of fifteen Wistar rats induced via 3,4-benzo(α)pyrene and in tumor bearing Wistar rats induced by inoculation of the same cells, were studied. Electromagnetic resonance frequencies measurements and exposure of cells and tumor-bearing animals were performed by a device called multi channel dynamic exciter 100 VI (MCDE). The LMS cell lines were exposed to electromagnetic resonance radio frequencies (ERFs) ranging between 10 KHz and 120 KHz for 45 min whilst the tumor-bearing animals were exposed for 45 min daily for two consecutive days. Leiomyosarcoma cells exposed to the same pattern of radio frequencies were inoculated in female Wistar rats. Another group of Wistar rats was inoculated with non-exposed to EMFs LMS cells (4x106 cells per animal). The animals were separated into three groups. The first group was sham-exposed to the resonant EMFs (control group, CG), the second group was exposed to non-resonant EMF radiation pattern for 5 hours per day for a maximum of 60 days after the appearance of a palpable mass (experimental control group-ECG). The third group was exposed to the resonant EMF radiation, for 5 hours for maximum 60 days after the appearance of a palpable mass to the inoculation site (Experimental group, EG-I). The fourth group of animals was inoculated with LMS cells exposed to resonance EMFs with no further exposure to electromagnetic fields (Experimental group II, EG-II). After 24 hours the LMS cells exposed to the resonant EMFs of incubation did not manifest any inhibition of cell proliferation but at the end of 48 hours of incubation, the proliferation was dramatically decreased by 98% (p<0.01). The 2% survived cells were exposed once again for 45 min (totally 4 sessions of exposure) to the resonant EMF fields. Flow cytometry revealed that 45% of these cells underwent apoptosis and only a small percent (2%) underwent mitosis. In order to determine their metastatic potential, these EMF cells were also counted and tested by an aggregometer for their ability to aggregate platelets. No difference in platelet aggregation was shown compared to the aggravating effects of non-exposed LMS cells. In animal groups, the tumor induction was 100% and histology revealed that all tumors were leiomyosarcomas. In the case of the EG-I group, a number of tumors were completely regretted (final tumor induction: 66%). Both groups of animals inoculated with exposed and non-exposed to the EMF cells (EG-I and EG-II) demonstrated a significant prolongation of their survival time and a significant decrease in tumor growth rate, in comparison to the control group (CG). However, the survival time of EG-I animals was found to be significantly longer and tumor growth rate significantly lower compared to the EG-II animals. Results as above indicate that application of resonant EMF waves to malignant tumor bearing animals and leiomyosarcoma cells induces significant anticancer effects. The effects are mainly attributed to apoptosis induction of malignant cells.
Alternative title / Subtitle: αποτελέσματα σε σειρές κακοήθων κυττάρων σαρκώματος και κακοήθων σαρκωματικών όγκων πειραματόζωων
their efficacy in the treatment of malignant cells and tumors
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Subject classification: Ηλεκτρομαγνητικό πεδίο
Σαρκώμα
Keywords: Λειομυοσάρκωμα,Ηλεκτρομαγνητικό κύμα,Κακοήθεια,Επίμυες,Ακτινοβολία,Συχνότητα συντονισμού,Leiomyosarcoma,Elecromagnetic wave,Cancer,Rats,Radiation,Resonant frequency
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/28972
Appears in Collections:Διδακτορικές Διατριβές

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