Synthesis, structural characterization and biological studies of novel mixed ligand Ag(I) complexes with triphenylphosphine and aspirin or salicylic acid (Journal article)

Poyraz, M./ Banti, C. N./ Kourkoumelis, N./ Dokorou, V./ Manos, M. J./ Simcic, M./ Golic-Grdadolnik, S./ Mavromoustakos, T./ Giannoulis, A. D./ Verginadis,, II/ Charalabopoulos, K./ Hadjikakou, S. K.

Two new mixed ligand silver(I) complexes of formulae {[Ag(tpp)(3)(asp)](dmf)} (1) (aspH = o-acetylsalicylic acid and tpp = triphenylphosphine) and [Ag(tpp)(2)(o-Hbza)] (2) (o-HbzaH = o-hydroxy-benzoic acid) were synthesized and characterized by elemental analyses, spectroscopic techniques and X-ray crystallography at ambient conditions. Three phosphorus and one carboxylic oxygen atoms from a de-protonated aspirin ligand in complex 1 and two phosphorus and two carboxylic oxygen atoms from a chelating o-Hbza anion in complex 2 form a tetrahedral geometry around Ag(I) ions in both complexes. Complexes 1 and 2 and the silver(I) nitrate, tpp, aspNa and o-HbzaH were tested for their in vitro cytotoxic activity against leiomyosarcoma cells (LMS), human breast adenocarcinoma cells (MCF-7) and normal human fetal lung fibroblasts (MRC-5) cells with Thiazolyl Blue Tetrazolium Bromide (MTT) assay. For both cell lines 1 and 2 were found to be more active than cisplatin. Additionally, 1 and 2 exhibit lower activity on cell growth proliferation of MRC-5 cells. The type of LMS cell death caused by 1 and 2 were evaluated in vitro by use of flow cytometry assay. The results show that at concentrations of 1.5 and 1.9 mu M of complex 1, 44.1% and 69.4%, respectively of LMS cells undergo programmed cell death (apoptosis). When LMS cells were treated with 1.6 and 2.3 mu M of 2, LMS cells death was by 29.6% and 81.3%, respectively apoptotic. Finally, the influence of the complexes 1 and 2, upon the catalytic peroxidation of linoleic acid to hydroperoxylinoleic acid by the enzyme lipoxygenase (LOX) was kinetically and theoretically studied. The binding of 1 and 2 towards LOX was also investigated by Saturation Transfer Difference (STD) H-1 NMR experiments. (C) 2011 Elsevier B.V. All rights reserved.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Bioinorganic chemistry,Silver(I) complexes,Aspirin,Crystal,structures,Cytotoxic activity,STD H-1 NMR,crystal-structure,silver(i) complexes,in-vitro,anticonvulsant,activities,derivatives,organotin(iv),lipoxygenase,thioamides,dinuclear,gold(i)
ISSN: 0020-1693
Link: <Go to ISI>://WOS:000293783900016
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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