The detection of glucocorticoid receptors in breast cancer by immunocytochemical and biochemical methods (Journal article)
Ioannidis, C./ Papamichail, M./ Agnanti, N./ Garas, J./ Tsawdaroglou, N./ Sekeris, C. E.
Glucocorticoid receptors have been detected in 90 human breast tumors and tumor-like conditions by the immunoperoxidase method using a specific antibody against the glucocorticoid receptor isolated from rat thymocytes. In some of the specimens the [3H]-dexamethasone binding assay was also performed and the results obtained were compared with those of the immunoperoxidase method. When the biochemical method was used, no strict correlation between the degree of binding of [3H]-dexamethasone and malignancy on the basis of histological findings could be demonstrated. In contrast, the immunoperoxidase method was in full agreement with the histological type of the tumor. Thus, nearly all malignant breast tumors (carcinomas) were positive by the immunoperoxidase method to a vary degree. Most of the examined benign tumor-like conditions (fibrocystic disease) were found to be negative. Intermediate situations, such as atypical duct of lobular hyperplasia, papillomatosis etc, were mostly positive. These findings suggest that ther immunoperoxidase method, part from its usefulness for the detection of glucocorticoid receptors in breast tissue, may be used as an early biological marker to detect early conversion of normal to hyperplastic tissue and/or malignancy of the mammary gland.
|Institution and School/Department of submitter:||Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής|
|Keywords:||Breast Neoplasms/*metabolism/pathology,Dexamethasone/metabolism,Female,Fibrocystic Breast Disease/metabolism/pathology,Humans,Immunoenzyme Techniques,Receptors, Glucocorticoid/*analysis,Receptors, Steroid/*analysis|
|Appears in Collections:||Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)|
Files in This Item:
|Ioannidis-1982-The detection of glu.pdf||699.72 kB||Adobe PDF||View/Open Request a copy|
Please use this identifier to cite or link to this item:This item is a favorite for 0 people.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.