A collaborative evaluation of the cytotoxicity of two surfactants by using the human corneal epithelial cell line and the WST-1 test (Journal article)

Huhtala, A./ Alajuuma, P./ Burgalassi, S./ Chetoni, P./ Diehl, H./ Engelke, M./ Marselos, M./ Monti, D./ Pappas, P./ Saettone, M. F./ Salminen, L./ Sotiropoulou, M./ Tahti, H./ Uusitalo, H./ Zorn-Kruppa, M.

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dc.contributor.authorHuhtala, A.en
dc.contributor.authorAlajuuma, P.en
dc.contributor.authorBurgalassi, S.en
dc.contributor.authorChetoni, P.en
dc.contributor.authorDiehl, H.en
dc.contributor.authorEngelke, M.en
dc.contributor.authorMarselos, M.en
dc.contributor.authorMonti, D.en
dc.contributor.authorPappas, P.en
dc.contributor.authorSaettone, M. F.en
dc.contributor.authorSalminen, L.en
dc.contributor.authorSotiropoulou, M.en
dc.contributor.authorTahti, H.en
dc.contributor.authorUusitalo, H.en
dc.contributor.authorZorn-Kruppa, M.en
dc.rightsDefault Licence-
dc.subjectBenzalkonium Compounds/*poisoningen
dc.subjectCell Division/drug effectsen
dc.subjectCell Line, Transformeden
dc.subjectCell Survival/drug effectsen
dc.subjectCulture Media, Serum-Free/pharmacologyen
dc.subjectEndothelium, Corneal/cytology/*drug effects/physiologyen
dc.subjectPolyethylene Glycols/*poisoningen
dc.subjectSurface-Active Agents/*poisoningen
dc.subjectTime Factorsen
dc.titleA collaborative evaluation of the cytotoxicity of two surfactants by using the human corneal epithelial cell line and the WST-1 testen
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.abstractThis study was undertaken to investigate the use of the in vitro test WST-1, an assay of cell proliferation and viability, for a preliminary safety evaluation of topical ophthalmic preparations. The cytotoxicity of two surfactants, benzalkonium chloride (BAC) and polyoxyethylene-20-stearyl ether (Brij78, PSE) was independently investigated in four laboratories in the EU by using an immortalized human corneal epithelial (HCE) cell line. The HCE cells were exposed to BAC and PSE for 5 min, 15 min, and 1 hour, and the results of the HCE-WST-1 tests were collected and compared. After one-hour exposure, the EC(50) values in BAC-treated cells in the presence of serum ranged between 0.0650 +/- 0.0284 (mean +/- SD) mM, and those in the absence of serum 0.0296 +/- 0.0081 mM. The corresponding values for PSE were 0.0581 +/-.0300 mM and 0.0228 +/-.0063 mM. There were variations in the results between different laboratories, with coefficients of variation ranging from 31 to 121%, mean 58%. The use of one-hour exposure time is to be preferred, and the elimination of serum in the culture medium is recommended to avoid both underestimation of toxic effects and variability of the test results.en
heal.journalNameJ Ocul Pharmacol Theren
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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