Absence of HCV viraemia in anti-HCV-negative haemodialysis patients (Journal article)

Dalekos, G. N./ Boumba, D. S./ Katopodis, K./ Zervou, E./ Sferopoulos, G./ Elisaf, M. S./ Tsianos, E. V./ Siamopoulos, K. C.


BACKGROUND: Immunologic alterations have been reported in chronic haemodialysis (HD) patients. Some HD patients may have, therefore, an inability to produce detectable amounts of serum antibodies to hepatitis C virus (anti-HCV). Previous studies have shown the presence of HCV viraemia in anti-HCV-negative HD patients (ranging from 1 to 15%). However, the universal epidemiologic impact of these cases remains uncertain since there are conflicting results. In this context, we conducted a study in an attempt to investigate the presence of HCV viraemia among anti-HCV-negative HD patients in a well-defined geographic area of the northwestern part of Greece. METHODS: During a 6 month period, 81 anti-HCV-negative HD patients were tested twice for the presence of HCV RNA, using the reverse transcriptase polymerase chain reaction (RT-PCR) combined with a DNA enzyme immunoassay (DEIA). At the same time, periodic testing for anti-HCV by two commercially available third generation assays was done. In addition, 15 anti-HCV-positive HD patients and 20 non-HD patients with well established chronic HCV infection used as internal controls were tested for the presence of HCV RNA and anti-HCV. RESULTS: None of the anti-HCV-negative HD patients were shown to be viraemic by the combined RT-PCR and DEIA method. During the same time period, all remained anti-HCV negative by the third generation assays. By contrast, all the patients with known HCV-infection were positive by the two enzyme immunoassays, whereas 13 anti-HCV-positive HD patients (86.7%) and 18 non-HD patients (90%) were viraemic by RT-PCR. CONCLUSIONS: This study demonstrated that routine HCV RNA testing in anti-HCV-negative HD patients appears not to be necessary particularly when third generation assays are used for the detection of anti-HCV.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Adolescent,Adult,Aged,Female,Hepacivirus/genetics/*isolation & purification,Hepatitis Antibodies/*blood,Hepatitis C/*diagnosis/immunology/virology,Humans,Immunoenzyme Techniques,Male,Middle Aged,Polymerase Chain Reaction,RNA, Viral/blood/genetics,Renal Dialysis/*adverse effects,Viremia/*diagnosis/immunology/virology
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/24167
ISSN: 0931-0509
Link: http://www.ncbi.nlm.nih.gov/pubmed/9681732
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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