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  4. Τμήμα Ιατρικής
  5. Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ
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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/24058
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dc.contributor.authorLakka, S. S.en
dc.contributor.authorRajagopal, R.en
dc.contributor.authorRajan, M. K.en
dc.contributor.authorMohan, P. M.en
dc.contributor.authorAdachi, Y.en
dc.contributor.authorDinh, D. H.en
dc.contributor.authorOlivero, W. C.en
dc.contributor.authorGujrati, M.en
dc.contributor.authorAli-Osman, F.en
dc.contributor.authorRoth, J. A.en
dc.contributor.authorYung, W. K.en
dc.contributor.authorKyritsis, A. P.en
dc.contributor.authorRao, J. S.en
dc.date.accessioned2015-11-24T19:37:38Z-
dc.date.available2015-11-24T19:37:38Z-
dc.identifier.issn1078-0432-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/24058-
dc.rightsDefault Licence-
dc.subjectAdenoviridae/geneticsen
dc.subjectAnimalsen
dc.subjectCarcinoma, Non-Small-Cell Lung/*drug therapy/secondaryen
dc.subjectDNA, Antisense/genetics/pharmacology/*therapeutic useen
dc.subjectFemaleen
dc.subjectGene Transfer Techniquesen
dc.subjectGenetic Vectorsen
dc.subjectLung Neoplasms/*drug therapy/pathologyen
dc.subjectMiceen
dc.subjectNeoplasm Invasivenessen
dc.subjectNeoplasm Transplantationen
dc.subjectProtein Biosynthesis/drug effectsen
dc.subjectRNA, Messenger/drug effects/metabolismen
dc.subjectReceptors, Cell Surface/*antagonists & inhibitors/genetics/metabolismen
dc.subjectReceptors, Urokinase Plasminogen Activatoren
dc.subjectTumor Cells, Cultureden
dc.subjectXenograft Model Antitumor Assaysen
dc.titleAdenovirus-mediated antisense urokinase-type plasminogen activator receptor gene transfer reduces tumor cell invasion and metastasis in non-small cell lung cancer cell linesen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/11309361-
heal.identifier.secondaryhttp://clincancerres.aacrjournals.org/content/7/4/1087.full.pdf-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate2001-
heal.abstractThe urokinase-type plasminogen activator (uPA) and its receptor (uPAR) play an important role in the proteolytic cascade involved in the metastasis of lung and other cancers. We report that the reduction in uPAR levels produced by an antisense strategy using an adenovirus construct (Ad-uPAR) in H1299 cells, an invasive human lung cancer cell line that produces high levels of uPAR, resulted in a decrease of uPAR levels to 80-90% of those seen in cells infected with mock or adenovirus (Ad)-cytomegalovirus vector controls. In addition, increasing the multiplicity of infection from 25 to 200 caused a corresponding decrease in the level of uPAR protein within 5 days of treatment, as shown by Western blot analysis. Furthermore, the in vitro translation of total RNA levels of Ad-uPAR-infected H1299 cells in a rabbit reticulocyte lysate system caused a 50-70% decrease in uPAR immunoprecipitate in Ad-uPAR-infected cells relative to the levels in cells of mock and vector controls. The Matrigel invasion assay showed the invasion of H1299 cells and A549 cells infected with Ad-uPAR to be decreased by 70% relative to mock- and vector-infected controls. Infection of tumor cells with Ad-uPAR before implantation significantly reduced the incidence of lung metastasis by 85% as compared with the control virus-infected cells injected into nude mice through the tail vein. Our collective results show that the uPAR system is a potential target of treatment for lung cancers.en
heal.journalNameClin Cancer Resen
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
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