An in vivo study on the synchronizing effect of hydroxyurea (Journal article)

Maurer-Schultze, B./ Siebert, M./ Bassukas, I. D.

The effect of hydroxyurea (HU; 0.5 mg/g body wt) on L 1210 ascites tumor cells has been studied using various cell kinetic methods. In contrast to the general assumption that HU blocks cells at the G1/S boundary [J. Brachet (1985) Molecular Cytology, Vol. I, p. 266, Academic Press, New York], the present results show that the cells are not held at G1/S but enter S at about the normal rate and are accumulated in early S phase due to a dose-dependent inhibiting effect of HU on DNA synthesis. Partial synchronization of the cells demonstrated by a distinct mitotic peak 10 h after HU application is not due to a G1/S block of the cells and their subsequent synchronous passage through the cycle after release from the block but is due to rather complex mechanisms of action of HU: a differential cytocidal effect and an effect on the passage of the cells through the cycle, both depending on the position of the cells throughout the cycle. HU kills S-phase cells, mainly cells in early S phase; i.e., a great portion of the cells "accumulated" in early S phase is killed by the drug, while G1-phase cells are almost not affected by the lethal effect of HU. These G1-phase cells pass through the cycle more rapidly after cessation of the HU effect. The same is true for the surviving cells accumulated in early S phase, while part of the cells in the remaining S phase are delayed in their passage through the cycle. This causes partial synchronization, since a great portion of all cells that survive HU treatment reach mitosis at the same time.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Animals,Autoradiography,Carbon Radioisotopes,Cell Cycle/*drug effects,Cell Division/drug effects,DNA Replication/*drug effects,Female,Hydroxyurea/*pharmacology,Kinetics,Leukemia L1210/*pathology,Mice,Mice, Inbred Strains,Thymidine/metabolism,Tritium
URI: https://olympias.lib.uoi.gr/jspui/handle/123456789/23890
ISSN: 0014-4827
Link: http://www.ncbi.nlm.nih.gov/pubmed/3335224
http://ac.els-cdn.com/0014482788901577/1-s2.0-0014482788901577-main.pdf?_tid=2f513bb7456d5604e612c2ee7f3bcd01&acdnat=1333701127_c277e511481dd03b1c38ceb815414595
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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