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  5. Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ
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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/23717
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dc.contributor.authorKurg, A.en
dc.contributor.authorTonisson, N.en
dc.contributor.authorGeorgiou, I.en
dc.contributor.authorShumaker, J.en
dc.contributor.authorTollett, J.en
dc.contributor.authorMetspalu, A.en
dc.date.accessioned2015-11-24T19:35:35Z-
dc.date.available2015-11-24T19:35:35Z-
dc.identifier.issn1090-6576-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/23717-
dc.rightsDefault Licence-
dc.subjectDNA/chemistry/*geneticsen
dc.subjectDNA Mutational Analysis/instrumentation/*methodsen
dc.subjectDNA Primers/*chemistryen
dc.subjectEvaluation Studies as Topicen
dc.subjectFluorescenceen
dc.subjectFluorescent Dyesen
dc.subjectGenetic Testing/instrumentation/methodsen
dc.subjectHeterozygote Detectionen
dc.subjectHumansen
dc.subjectMicrochemistry/instrumentation/methodsen
dc.subjectNucleic Acid Hybridization/methodsen
dc.subjectOligonucleotide Array Sequence Analysis/instrumentation/*methodsen
dc.subjectPolymerase Chain Reaction/methodsen
dc.subjectReproducibility of Resultsen
dc.subjectSensitivity and Specificityen
dc.subjectbeta-Thalassemia/diagnosis/*geneticsen
dc.titleArrayed primer extension: solid-phase four-color DNA resequencing and mutation detection technologyen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.primary10.1089/109065700316408-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/10794354-
heal.identifier.secondaryhttp://online.liebertpub.com/doi/pdfplus/10.1089/109065700316408-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate2000-
heal.abstractThe technology and application of arrayed primer extension (APEX) is presented. We describe an integrated system with DNA chip and template preparation, multiplex primer extension on the array, fluorescence imaging, and data analysis. The method is based upon an array of oligonucleotides, immobilized via the 5' end on a glass surface. A patient DNA is amplified by PCR, digested enzymatically, and annealed to the immobilized primers, which promote sites for template-dependent DNA polymerase extension reactions using four unique fluorescently labeled dideoxy nucleotides. A mutation is detected by a change in the color code of the primer sites. The technology was applied to the analysis of 10 common beta-thalassemia mutations. Nine patient DNA samples, each of which carries a different mutation, and four wild-type DNA samples were correctly identified. The signal-to-noise ratio of this technology is, on the average, 40:1, which enables the identification of heterozygous mutations with a high confidence level. The APEX method can be applied to any DNA target for efficient analysis of mutations and polymorphisms.en
heal.journalNameGenet Testen
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
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