Beta 2-microglobulin and HLA-DR expression in relation to the presence of Epstein-Barr virus in nasopharyngeal carcinomas (Journal article)

Kouvidou, C./ Rontogianni, D./ Tzardi, M./ Datseris, G./ Panayiotides, I./ Darivianaki, K./ Karidi, E./ Delides, G./ Kanavaros, P.

We have investigated the immunohistochemical expression of beta 2-microglobulin and HLA-DR proteins in nasopharyngeal carcinoma (NPC) in relation to the expression of the Epstein-Barr virus (EBV)-encoded EBER 1-2 mRNAs and LMP-1 protein. beta 2-Microglobulin is expressed in association with MHC-I molecules on most nucleated cells and HLA-DR belongs to the MHC-II molecules which are expressed mostly on antigen-presenting cells. Formalin-fixed paraffin-embedded tissue from 37 NPC cases was stained with immunohistochemistry for beta 2-microglobulin, HLA-DR and LMP-1 proteins and by RNA in situ hybridization for EBER 1-2 mRNAs. beta 2-Microglobulin-positive staining was found in neoplastic cells in all cases. In 19/37 NPC cases the positive staining was found in most neoplastic cells. In the remaining 18 NPC cases more than 25% of the neoplastic cells showed significantly reduced or negative staining in comparison to the normal epithelium and infiltrating small lymphocytes. HLA-DR-positive staining was found in 27/37 NPC cases. EBER 1-2 transcripts were deteced in neoplastic cells in 13/37 NPC cases. LMP-1 expression in tumour cells was found in 4/13 EBER1-2-positive cases. No correlation was found between the presence of EBER1-2 transcripts or LMP-1 protein and the beta 2-microglobulin-reduced expression in NPC. Thus, EBV does not seem to use downregulation of MHC-I to avoid the T cell cytotoxic immune response in NPC. HLA-DR expression was observed in all 13 EBV-positive cases of NPC, suggesting that EBV may participate in the induction of HLA-DR expression in a proportion of NPC.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Adult,Aged,Aged, 80 and over,Carcinoma/immunology/virology,Carcinoma, Squamous Cell/*immunology/*virology,Female,HLA-DR Antigens/*metabolism,Herpesvirus 4, Human/genetics/*isolation & purification,Humans,Immunohistochemistry,In Situ Hybridization,Male,Middle Aged,Nasopharyngeal Neoplasms/*immunology/*virology,RNA, Viral/analysis/genetics,RNA-Binding Proteins/genetics,*Ribosomal Proteins,Viral Matrix Proteins/metabolism,beta 2-Microglobulin/*metabolism
ISSN: 1015-2008
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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